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MABF1647 Anti-CD19 Antibody, FITC Conjugated, (human), clone SJ25C1

This mouse monoclonal Anti-CD19 (human), FITC, clone SJ25C1, Cat. No. MABF1647 is validated for use in Flow Cytometry, for the detection of CD19.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: CD19 UniProt Number: P15391
MABF1647
25 tests  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMFITCMonoclonal Antibody
Description
Catalogue NumberMABF1647
DescriptionAnti-CD19 Antibody, FITC Conjugated, (human), clone SJ25C1
Alternate Names
  • B-lymphocyte antigen CD19
  • B-lymphocyte surface antigen B4
  • Differentiation antigen CD19
  • T-cell surface antigen Leu-12
  • CD antigen CD19
Background InformationThe SJ25C1 antibody reacts with human CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21 (CR2), CD81 (TAPA-1) and CD225 (Leu13), in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. Upon activation and tyrosine phosphorylation, the CD19 molecule can provide an anchor for cytoplasmic signaling proteins such as GRB2, SOS or PLCG2. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells.

The SJ25C1 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.
Product Information
FormatFITC
PresentationPurified mouse monoclonal in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-CD19 (human), FITC, clone SJ25C1, Cat. No. MABF1647 is validated for use in Flow Cytometry, for the detection of CD19.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneSJ25C1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • CD19
Purification MethodPurified by size-exclusion chromatography
UniProt Number
Product Usage Statements
Quality AssuranceThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Evaluated by Flow Cytometry in human peripheral blood lymphocytes.

Flow Cytometry Analysis (FC): 0.5 μg of this antibody detected CD19 in one million human peripheral blood lymphocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size25 tests
Global Trade Item Number
Catalogue Number GTIN
MABF1647 04055977289619