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AB1846 Anti-C-X-C Chemokine Receptor 4 Antibody, NT

AB1846
100 µg  
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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IP, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB1846
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-C-X-C Chemokine Receptor 4 Antibody, NT
Alternate Names
  • CD184
  • CXCR4
Product Information
FormatPurified
Control
  • Skin
PresentationPurified IgG in PBS containing 0.02% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-C-X-C Chemokine Receptor 4 Antibody, N-terminus is an antibody against C-X-C Chemokine Receptor 4 for use in IC, IP & WB.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern blot: 1-2 μg/mL. HeLa cell lysates can be used as positive control. The antibody should detect a band at 40 kDa.

Immunocytochemistry: 10 μg/mL

Immunoprecipitation

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenAmino-terminus peptide of human CXCR4 (Berson, 1996; Leotscher, 1994).
EpitopeN-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes CXCR4, N-terminus.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Gene Symbol
  • CXCR4
  • WHIM
  • NPY3R
  • NPYY3R
  • FB22
  • LAP3
  • Fusin
  • CXCR-4
  • LCR1
  • NPYR
  • HM89
  • fusin
  • HSY3RR
  • CXC-R4
  • NPYY3
  • NPYRL
  • D2S201E
  • CD184
  • LESTR
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P61073 # Receptor for the C-X-C chemokine CXCL12/SDF-1. Transduces a signal by increasing the intracellular calcium ions level. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.
SIZE: 352 amino acids; 39746 Da
SUBUNIT: Interacts with HIV-1 surface protein gp120 and Tat.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
DOMAIN: SwissProt: P61073 The amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity.
PTM: Sulfated.
DISEASE: SwissProt: P61073 # Defects in CXCR4 are a cause of WHIM syndrome [MIM:193670]; also called warts, hypogammaglobulinemia, infections, and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
Molecular Weight40 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain 1 year at 4°C, from date of shipment. Do not freeze. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
AB1846 04053252368936