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203637
Sigma-AldrichAnti-Bone Sialoprotein Mouse mAb (ID1.2)
Anti-Bone Sialoprotein, mouse monoclonal, clone ID1.2, recognizes ~70-80 kDa bone sialoprotein in osteoblasts and osteocytes. It is validated for ELISA, Western blotting, and for paraffin sections.
More>>Anti-Bone Sialoprotein, mouse monoclonal, clone ID1.2, recognizes ~70-80 kDa bone sialoprotein in osteoblasts and osteocytes. It is validated for ELISA, Western blotting, and for paraffin sections. Less<<
Anti-Bone Sialoprotein Mouse mAb (ID1.2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ELISA (see comments) Immunoblotting (1:1000) Paraffin Sections (see comments)
Application Comments
Does not cross-react with human osteocalcin, osteonectin, osteopontin, or bone alkaline phosphatase. This antibody has also been reported to work for ELISA and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
purified human bone sialoprotein
Immunogen
Human
Clone
ID1.2
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute with dH₂O. Please see label for lot-specific reconstitution volume.
Mercury prohibited
To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
Anti-Bone Sialoprotein Mouse mAb (ID1.2) Certificates of Analysis
Title
Lot Number
203637
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
27-June-2007 RFH
Application
ELISA (see comments) Immunoblotting (1:1000) Paraffin Sections (see comments)
Description
Purified mouse monoclonal antibody. Recognizes the ~70-80 kDa bone sialoprotein.
Background
Except for osteocalcin and osteonectin, bone sialoprotein (BSP) is one of the major noncollagenous proteins in the extracellular matrix of bone. BSP could be useful as a potential serum marker for monitoring bone remodeling in diseases concerning bone turnover, pathological bone alterations and the rapid increase of osteoporosis.
Host
Mouse
Immunogen species
Human
Immunogen
purified human bone sialoprotein
Clone
ID1.2
Isotype
IgG₁
Species
human
Positive control
Human osteoblasts or osteocytes
Form
Lyophilized
Formulation
Lyophilized from 50 mM Tris, pH 7.4.
Preservative
0.04% Thimerosal, ≤0.1% sodium azide
Comments
Does not cross-react with human osteocalcin, osteonectin, osteopontin, or bone alkaline phosphatase. This antibody has also been reported to work for ELISA and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute with dH₂O. Please see label for lot-specific reconstitution volume.
