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NE1004 Anti-Apolipoprotein E Mouse mAb (E6D7)

Anti-Apolipoprotein E, mouse monoclonal, clone E6D7, recognizes the ~35 kDa Apo E2, E3, and E4 in human serum and brain. It is validated for ELISA, WB, IP, and paraffin and frozen sections.

Anti-Apolipoprotein E Mouse mAb (E6D7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-ApoE

NE1004
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
NE1004-100UL Plastic ampoule 100 ul
Description
OverviewRecognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum and brain.
Catalogue NumberNE1004
Brand Family Calbiochem®
SynonymsAnti-ApoE
Application Data
Detection of human apolipoprotein E by immunoblotting. Sample: Human serum (1 μl). Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: chemiluminescence.

Detection of human apolipoprotein E by staining paraffin sections. Sample: Vascular amyliod β Down's Syndrome brain tissue. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB.

Detection of human apolipoprotein E by staining paraffin sections. Sample: Alzheimer's disease brain tissue containing senile plaques. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB
References
ReferencesGolabek, A.A., et al. 1996. J. Biol. Chem. 271, 10602.
Product Information
FormLiquid
FormulationCulture supernatant diluted in PBS.
Positive controlBrain tissue, serum
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1:100-1:1000)
Immunoprecipitation (see comments)
Frozen Sections (1:100-1:10,000)
Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)
Application CommentsRecognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE
ImmunogenHuman
CloneE6D7
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NE1004-100UL 04055977209839

Documentation

Anti-Apolipoprotein E Mouse mAb (E6D7) SDS

Title

Safety Data Sheet (SDS) 

Anti-Apolipoprotein E Mouse mAb (E6D7) Certificates of Analysis

TitleLot Number
NE1004

References

Reference overview
Golabek, A.A., et al. 1996. J. Biol. Chem. 271, 10602.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-October-2010 JSW
SynonymsAnti-ApoE
ApplicationELISA (see comments)
Immunoblotting (1:100-1:1000)
Immunoprecipitation (see comments)
Frozen Sections (1:100-1:10,000)
Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)
Application Data
Detection of human apolipoprotein E by immunoblotting. Sample: Human serum (1 μl). Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: chemiluminescence.

Detection of human apolipoprotein E by staining paraffin sections. Sample: Vascular amyliod β Down's Syndrome brain tissue. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB.

Detection of human apolipoprotein E by staining paraffin sections. Sample: Alzheimer's disease brain tissue containing senile plaques. Primary antibody: Anti-Apolipoprotein E Mouse mAb (E6D7) (Cat. No. NE1004). Detection: DAB
DescriptionMouse monoclonal antibody supplied as diluted culture supernatant. Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E.
BackgroundApolipoprotein E (Apo E) is a polymorphic, multifunctional protein synthesized by liver, kidney, brain, and other cell types. It has been reported that the genetic inheritance of apolipoprotein isoform E4 is a significant risk factor for late onset Alzheimer's Disease (AD).
HostMouse
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE
CloneE6D7
IsotypeIgG₁
Specieshuman, not mouse, not rabbit
Positive controlBrain tissue, serum
FormLiquid
FormulationCulture supernatant diluted in PBS.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsRecognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesGolabek, A.A., et al. 1996. J. Biol. Chem. 271, 10602.