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ABE316 Anti-ARID1B Antibody

This Anti-ARID1B Antibody is validated for use in Western Blotting, Immunoprecipitation for the detection of ARID1B.

Anti-ARID1B Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: ARID1B, BAF250B, DAN15, KIAA1235, OSA2 UniProt Number: Q8NFD5
ABE316
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE316
DescriptionAnti-ARID1B Antibody
Alternate Names
  • AT-rich interactive domain-containing protein 1B
  • ARID domain-containing protein 1B
  • BRG1-associated factor 250b
  • BAF250B
  • BRG1-binding protein hELD/OSA1
  • Osa homolog 2
  • hOsa2
  • p250R
Background InformationARID1B (AT rich interactive domain 1B), also known as BAF250B, p250R and hOsa2, is a nuclear protein and a member of the SWI/SNF-A chromatin-remodeling complex. It is involved in repression of select genes by chromatin remodeling and transcriptional activation. It non-specifically binds DNA and alters DNA nucleosome topology. The cause of mental retardation autosomal dominant type 12 (MRD12) are defects in ARID1B. High levels of ARID1B can be found in heart, skeletal muscle and kidney.
References
Product Information
FormatAffinity Purified
Control
  • Raji cell nuclear extract
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-ARID1B Antibody is validated for use in Western Blotting, Immunoprecipitation for the detection of ARID1B.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated ARID1B from Raji cell nuclear extract.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human ARID1B.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ARID1B
  • BAF250B
  • DAN15
  • KIAA1235
  • OSA2
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~250 kDa observed. Uncharacterized bands may be observed at ~16 kDa and ~32 kDa in some lysates.. Uniprot describes 4 isoforms produced by alternative splicing at ~236 kDa, ~237 kDa, ~241 kDa and ~162 kDa. This antibody will recognize all isoforms.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in Raji cell nuclear extract.

Western Blot Analysis: 1.0 µg/mL of this antibody detected ARID1B in 10 µg of Raji cell nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE316 04053252826511