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ST1507 Anti-AKR1B10 Mouse mAb (1A6)

ST1507
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
ST1507-100UG 100 μg
Description
OverviewRecognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue.
Catalogue NumberST1507
Brand Family Calbiochem®
SynonymsAnti-Aldo-Keto Reductase 1B10
Application Data
Detection of human AKR1B10 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human AKR1B10 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon tissue. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (3 µg/ml). Detection: colorimetric (DAB).

Detection of human AKR1B10 by immunocytochemistry. Sample: HeLa cellsfixed in Paraformaldehyde. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (10 µg/ml). Detection: fluorescence.
References
ReferencesAhn K.H., et al. 2010 JOURNAL OF HEALTH SCIENCE 56, 287.
Satow, R., et al. 2010. Clin. Cancer Res. 16, 2518.
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Positive controlHepG2 cells, Human Colon tissue, HeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 76-144 of human AKR1B10, expressed as a GST fusion protein
ImmunogenNA
Clone1A6
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
ST1507-100UG 04055977224153