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NA81 Anti-5-Methylcytosine Mouse mAb (162 33 D3)

This Anti-5-Methylcytosine Mouse mAb (162 33 D3) is validated for use in ELISA, FC, Frozen Sections, IF, Paraffin Sections, Radioimmunoassay, Southwestern Blot for the detection of 5-Methylcytosine.

Anti-5-Methylcytosine Mouse mAb (162 33 D3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-5-mc, Anti-5-MeCyd

NA81
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
NA81-50UG Plastic ampoule 50 μg
Description
OverviewRecognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells.

Specific
• Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications
• Flow Cytometry
• Frozen Sections
• Immunoblotting
• Immunofluorescence
• Paraffin Sections
Reliable
• Generates reproducible results.
• Clone 162 33 D3 has been used in over 50 publications.

Catalogue NumberNA81
Brand Family Calbiochem®
SynonymsAnti-5-mc, Anti-5-MeCyd
Application Data
Detection of 5-methylcytosine by immunohistochemistry. Sample: Normal colon (left) and colon adenocarcinoma (right). Anti-5-Methylcytosine Mouse mAb (162 33 D3) (Cat. No. NA81). Detection: DAB

Chromosome methylation patterns of mouse embryos at the one-cell stage. Methylated sites were revealed by indirect immunofluorescence labeling with Anti-5-methylcytosine.
References
ReferencesEhrlich, M. 2002. Oncogene 21, 5400. (Review)
Widschwendter, M. and Jones, P.A. 2002. Oncogene 21, 5462. (Review)
Barton, S.C., et al. 2001. Hum. Mol. Genet. 10, 2983.
Piyathilake, C.J., et al. 2001. Hum. Pathol. 32, 856.
Taddei, A., et al. 2001. Nat. Cell Biol. 3, 114.
Piyathilake, C.J., et al. 2000. Biotech. Histochem. 75, 251.
De Capoa, A., et al. 1999. FASEB J. 13, 89.
Product Information
FormLiquid
FormulationIn PBS, pH 7.4.
Positive controlNIH3T3 cells
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesParaffin Sections Kang, J.S., et al. 2006. Cancer Sci. 97, 453.
Key Applications Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Paraffin Sections
Radioimmunoassay
Southwestern Blot
Application NotesELISA (0.1 µg/ml)
Flow Cytometry (1 µg/ml)
Frozen Sections (5-10 µg/ml)
Immunoblotting (5 µg/ml)
Immunofluorescence (1 µg/ml)
Paraffin Sections (5-10 µg/ml)
Radioimmunoassay (0.1 µg/ml)
Southwestern Blot (5 µg/ml)
Application CommentsClone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems.
Biological Information
Immunogen5-methylcytosine conjugated to ovalbumin
Clone162 33 D3
HostMouse
IsotypeIgG₁
Species Reactivity
  • A Broad Range Of Species
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NA81-50UG 04055977209792

Documentation

Anti-5-Methylcytosine Mouse mAb (162 33 D3) SDS

Title

Safety Data Sheet (SDS) 

Anti-5-Methylcytosine Mouse mAb (162 33 D3) Certificates of Analysis

TitleLot Number
NA81

References

Reference overview
Ehrlich, M. 2002. Oncogene 21, 5400. (Review)
Widschwendter, M. and Jones, P.A. 2002. Oncogene 21, 5462. (Review)
Barton, S.C., et al. 2001. Hum. Mol. Genet. 10, 2983.
Piyathilake, C.J., et al. 2001. Hum. Pathol. 32, 856.
Taddei, A., et al. 2001. Nat. Cell Biol. 3, 114.
Piyathilake, C.J., et al. 2000. Biotech. Histochem. 75, 251.
De Capoa, A., et al. 1999. FASEB J. 13, 89.

Brochure

Title
Antibody Sourcebookﾙ Sixth Edition
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-August-2008 JSW
SynonymsAnti-5-mc, Anti-5-MeCyd
ApplicationELISA (0.1 µg/ml)
Flow Cytometry (1 µg/ml)
Frozen Sections (5-10 µg/ml)
Immunoblotting (5 µg/ml)
Immunofluorescence (1 µg/ml)
Paraffin Sections (5-10 µg/ml)
Radioimmunoassay (0.1 µg/ml)
Southwestern Blot (5 µg/ml)
Application Data
Detection of 5-methylcytosine by immunohistochemistry. Sample: Normal colon (left) and colon adenocarcinoma (right). Anti-5-Methylcytosine Mouse mAb (162 33 D3) (Cat. No. NA81). Detection: DAB

Chromosome methylation patterns of mouse embryos at the one-cell stage. Methylated sites were revealed by indirect immunofluorescence labeling with Anti-5-methylcytosine.
DescriptionImmunoaffinity purified mouse monoclonal antibody. Recognizes 5-methylcytosine.
BackgroundDNA methylation plays an important role in gene regulation. Methylation of gene-promoter regions leads to loss of function while DNA demethylation can lead to gain of function. Alterations in DNA methylation may play an important role in carcinogenesis. DNA methylation varies according to tissue type. In some cancers types such as Wilms tumor and colon adenocarcinoma have a decrease in global DNA methylation. In contrast other cancers such as breast cancer have DNA hypermethylation. Changes in global DNA methylation status may be useful early markers for the detection of premalignant lesions.
HostMouse
Immunogen5-methylcytosine conjugated to ovalbumin
Clone162 33 D3
IsotypeIgG₁
Speciesa broad range of species
Positive controlNIH3T3 cells
FormLiquid
FormulationIn PBS, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsClone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesEhrlich, M. 2002. Oncogene 21, 5400. (Review)
Widschwendter, M. and Jones, P.A. 2002. Oncogene 21, 5462. (Review)
Barton, S.C., et al. 2001. Hum. Mol. Genet. 10, 2983.
Piyathilake, C.J., et al. 2001. Hum. Pathol. 32, 856.
Taddei, A., et al. 2001. Nat. Cell Biol. 3, 114.
Piyathilake, C.J., et al. 2000. Biotech. Histochem. 75, 251.
De Capoa, A., et al. 1999. FASEB J. 13, 89.
Application referencesParaffin Sections Kang, J.S., et al. 2006. Cancer Sci. 97, 453.