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14-933 ATM, active

ATM, active MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-933
10 µg  Also available in 250μg size as catalog # 14-933M and in bulk as catalog # 14-933-K.
Purchase on Sigma-Aldrich

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Overview

Replacement Information

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This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Description
Catalogue Number14-933
Trade Name
  • Upstate
DescriptionATM, active
OverviewATM is a serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation.
References
Product Information
PresentationProtein in frozen storage buffer
Quality LevelMQ100
Applications
Key Applications
  • Kinase Assay
Biological Information
SourceRecombinant, human FLAG-tagged ATM full length, expressed in a mammalian cell line.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • ATM
  • ATA
  • ATC
  • ATD
  • ATDC
  • TEL1
  • TELO1
  • AT1
  • ATE
UniProt Number
Molecular Weight352 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of recombinant human p53, full length (c-Myc, 6His tagged) as the substrate (Millipore cat # 23-034)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250μg size as catalog # 14-933M and in bulk as catalog # 14-933-K.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-933 04053252933691

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-933M ATM, active

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Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases