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14-614M ALK4 Protein, active, 250 µg

Active, N-terminal GST-tagged, recombinant, human ALK4 amino acids 150-end. For use in Kinase Assays.

14-614M
250 µg  Also available in 10 μg size (cat#14-614) and in bulk (cat#14-614-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-614M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionALK4 Protein, active, 250 µg
OverviewN-terminal GST-tagged, recombinant, human ALK4 amino acids 150-end.
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal GST-tagged, recombinant, human ALK4 amino acids 150-end. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHuman ALK4 expressed by baculovirus in Sf21 insect cells.
Entrez Gene Number
Entrez Gene SummaryActivins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with a cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling, and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. This gene encodes activin A type IB receptor, composed of 11 exons. Alternative splicing and alternative polyadenylation result in 3 fully described transcript variants. The mRNA expression of variants 1, 2, and 3 is confirmed, and a potential fourth variant contains an alternative exon 8 and lacks exons 9 through 11, but its mRNA expression has not been confirmed.
Gene Symbol
  • ACVR1B
  • ALK-4
  • ACVRLK4
  • ALK4
  • SKR2
  • ActRIB
  • ACTR-IB
  • ACTRIB
Protein TargetALK4
Purification MethodGlutathione-agarose
Target Sub-FamilyTKL
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P36896 # On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators.
COFACTOR: Magnesium or manganese (By similarity).
SIZE: 505 amino acids; 56807 Da
SUBUNIT: Interacts with AIP1. Part of a complex consisting of AIP1, ACVR2A, ACVR1B and SMAD3 (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.
SIMILARITY: SwissProt: P36896 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. & Contains 1 GS domain. & Contains 1 protein kinase domain.
Molecular Weight63.8kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of casein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C from date of shipment
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-614) and in bulk (cat#14-614-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-614M 04053252924316

Documentation

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Alternative Packsize

Catalogue Number Description
14-614 ALK4 Protein, active, 10 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases