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110630 AFB-Color malachite green (oxalate) solution

for the microscopic investigation of acid-fast bacteria (AFB) (cold staining)

AFB-Color malachite green (oxalate) solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
110630
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1106300500 500 ml
Description
Catalogue Number110630
DescriptionAFB-Color malachite green (oxalate) solution
OverviewAFB-Color malachite green (oxalate) solution - for the microscopic investigation of acid-fast bacteria (AFB) (cold staining), is a ready-to-use solution used for human-medical cell diagnosis and serves the purpose of the bacteriological investigation of sample material of human origin., for example smears of enriched bacterial cultures. Acid-fast bacteria are difficult to stain because of the high proportion of lipid and wax in their cell walls. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus the formation of the mycolate-fuchsin complex in the cell wall. In this case, a modification of the AFB-Color carbol fuchsin (Cat.no. 108512) solution renders heating unnecessary (Kinyoun method, cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use malachite green is used for counterstaining.
Pretreatment of the specimens with Sputofluol® (Cat. No. 108000) dissolves the bacteria from the surrounding viscid sputum and cell material.
The 500 ml bottle of AFB-Color malachite green (oxalate) solution provides 250 stainings. It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3204 13 00
Quality LevelMQ400
Applications
Applicationfor the microscopic investigation of acid-fast bacteria (AFB) (cold staining)
Biological Information
Physicochemical Information
Density1.0 g/cm3 (20 °C)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Storage class10 - 13 Other liquids and solids
WGKWGK 2 obviously hazardous to water
Disposal28
Aqueous solutions: Container D.
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Suitability for microscopypasses test
Acid-fast bacteriared
Backgroundlight green
Global Trade Item Number
Catalogue Number GTIN
1106300500 04022536126357

Documentation

AFB-Color malachite green (oxalate) solution SDS

Title

Safety Data Sheet (SDS) 

AFB-Color malachite green (oxalate) solution Certificates of Analysis

Product Number PackagingSpecificationLot Number
1106300500 PDF Specification Document