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SCM307 3dGRO™ Lung Organoid Branching Medium

Serum-free ready-to-use media used to differentiate anterior foregut endoderm (AFE) cells into lung bud organoids in suspension cultures.

SCM307
50 mL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCM307
Description3dGRO™ Lung Organoid Branching Medium
Alternate Names
  • Lung Bud Organoid Medium
  • Lung Organoid Differentiation Medium
Background InformationThe lung epithelium undergoes a complex series of endoderm–mesoderm-mediated signaling events to generate a complex arborized network of conducting airway (bronchi, bronchioles) and gas-exchanging units (alveoli). Lung epithelium differentiation stages include: anterior/posterior and dorsal/ventral patterning, lung specification, lung budding, branching morphogenesis, and maturation.
The 3dGRO™ Lung Organoid Branching Medium is a chemically defined/serum-free ready-to-use media used to differentiate Sox2+/Pax6+ anterior foregut endoderm (AFE) cells into lung bud organoids in suspension cultures. Additionally, the 3dGRO™ Lung Organoid Branching Medium can be used to culture AFE cells on fibronectin coated cultureware. The 3dGRO™ Lung Organoid Branching Medium is a component of the 3dGRO™ Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures. The 3dGRO™ Human Lung Organoid Culture System includes (all sold separately):

  • Definitive Endoderm Induction Medium (SCM302)
  • Anterior Foregut Endoderm Induction Medium I (SCM305)
  • Anterior Foregut Endoderm Induction Medium II (SCM306)
  • 3dGRO™ Lung Organoid Branching Medium (SCM307)
  • 3dGRO™ Lung Organoid Maturation Medium (SCM308)
  • Human iPSC Derived AFE Progenitors (SCC301)
References
Product Information
Applications
ApplicationSerum-free ready-to-use media used to differentiate anterior foregut endoderm (AFE) cells into lung bud organoids in suspension cultures.
Key Applications
  • Cell Culture
  • Stem Cell Culture
Biological Information
Media FormLiquid
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Appearance (Color): Clear/No Particlulates (Red Liquid)
• Osmolality: Pass
• pH: 7.0 – 7.4
• Sterility Tested: No Growth/Pass
• Endotoxin: <2 EU/mL
• Mycoplasma: Negative
• Functional Assay: Pass
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt, store at -20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly. Use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.
Packaging Information
Material Size50 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCM307 04061842079605