Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

506165 p53, His•Tag®, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Tumor Protein p53

View Products on Sigmaaldrich.com
506165
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, human, wild-type p53 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. p53, a tumor suppressor, blocks transformation and inhibits tumor cell growth. Numerous mutations in the p53 gene have been characterized in human cancers. Loss or mutation of p53 function is highly correlated with tumorigenesis. The purified protein is useful in DNA binding assays and as a protein kinase substrate.
Catalogue Number506165
Brand Family Calbiochem®
SynonymsTumor Protein p53
References
ReferencesLiu, G., et al. 2003. J. Biol. Chem. 278, 17557.
Zhang, L., et al. 2000. Cancer Res. 60, 3655.
Fields, S., and Jang, S.K. 1990. Science 249, 1046.
Michalovitz, D., et al. 1990. Cell 62, 671.
Raycroft, L., et al. 1990. Science 249, 1049.
Finlay, C.A., et al. 1989. Cell 57, 1083.
Product Information
FormLiquid
FormulationIn 50 mM KCl, 30 mM sodium pyrophosphate, 5% glycerol, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Biological activityTypical DNA binding assays require 0.5 ng to 2 ng of p53 protein per assay.
Purity≥80% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsProteases: none detected
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
506165 0

Documentation

p53, His•Tag®, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

p53, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
506165

References

Reference overview
Liu, G., et al. 2003. J. Biol. Chem. 278, 17557.
Zhang, L., et al. 2000. Cancer Res. 60, 3655.
Fields, S., and Jang, S.K. 1990. Science 249, 1046.
Michalovitz, D., et al. 1990. Cell 62, 671.
Raycroft, L., et al. 1990. Science 249, 1049.
Finlay, C.A., et al. 1989. Cell 57, 1083.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-March-2009 JSW
SynonymsTumor Protein p53
DescriptionRecombinant, human, wild-type p53 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. p53, a tumor suppressor, blocks transformation and inhibits tumor cell growth. Numerous mutations in the p53 gene have been characterized in human cancers. Loss or mutation of p53 function is highly correlated with tumorigenesis. The purified protein is useful in DNA binding assays and as a protein kinase substrate.
FormLiquid
FormulationIn 50 mM KCl, 30 mM sodium pyrophosphate, 5% glycerol, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥80% by SDS-PAGE
ContaminantsProteases: none detected
Biological activityTypical DNA binding assays require 0.5 ng to 2 ng of p53 protein per assay.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesLiu, G., et al. 2003. J. Biol. Chem. 278, 17557.
Zhang, L., et al. 2000. Cancer Res. 60, 3655.
Fields, S., and Jang, S.K. 1990. Science 249, 1046.
Michalovitz, D., et al. 1990. Cell 62, 671.
Raycroft, L., et al. 1990. Science 249, 1049.
Finlay, C.A., et al. 1989. Cell 57, 1083.