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595501 ThioGlo® 1 Fluorescent Thiol Reagent

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3H-Naphthol[2,1-b]pyran-s-carboxylic Acid, 10-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)-9-methoxy-3-oxo-, Methyl Ester

595501
  
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Overview

Replacement Information

Key Spec Table

CAS #Purity
137350-66-4≥99% by TLC
Description
Overview

This product has been discontinued.



We are offering Thiol Fluorescent Probe IV (Cat. No. 595504)as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






Maleimide reagent for the detection, determination, and derivatization of thiol groups using fluorescent techniques. Superior to other thiol modifying reagents due to high fluorescent quantum yields after reaction with SH (QYSH = 0.49), extremely low fluorescence from unreacted ThioGlo® Reagent (QYR = 0.008), fast reaction times (5000 times faster than bimanes), resistance to hydrolysis (t½ ≥8.5 hr at 25°C, pH 7.0), non-interfering hydrolysis products, minimal photobleaching, large Stokes shifts (134 nm), solvachromic properties, and high sensitivity (QYSH/QYR = 60).
Catalogue Number595501
Brand Family Calbiochem®
Synonyms3H-Naphthol[2,1-b]pyran-s-carboxylic Acid, 10-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)-9-methoxy-3-oxo-, Methyl Ester
References
ReferencesStorey, B.T., et al. 1998. Mol. Reprod. Dev. 49, 400.
Wright, S.K. and Viola, R.E. 1998. Anal. Biochem. 265, 8.
Langmuir, M.E., et al. 1996. in Fluorescence Microscopy and Fluorescent Probes (Slavic, J., ed.) pp. 229-233, Plenum Press, New York.
Product Information
CAS number137350-66-4
FormCrystalline solid
Hill FormulaC₂₀H₁₃NO₇
Chemical formulaC₂₀H₁₃NO₇
Structure formula ImageStructure formula Image
Applications
Biological Information
Purity≥99% by TLC
Physicochemical Information
Emission max.
Excitation max.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
595501 0

Documentation

ThioGlo® 1 Fluorescent Thiol Reagent Certificates of Analysis

TitleLot Number
595501

References

Reference overview
Storey, B.T., et al. 1998. Mol. Reprod. Dev. 49, 400.
Wright, S.K. and Viola, R.E. 1998. Anal. Biochem. 265, 8.
Langmuir, M.E., et al. 1996. in Fluorescence Microscopy and Fluorescent Probes (Slavic, J., ed.) pp. 229-233, Plenum Press, New York.

Citations

Title
  • Yusheng Han, et al. (2005) Ethacrynic Acid Inhibits Multiple Steps in the NF-κB Signaling Pathway. Shock 23, 45-53.
  • John R. Pawloski, Douglas T. Hess and Jonathan S. Stamler. (2005) Impaired vasodilation by red blood cells in sickle cell disease. Proceedings of the National Academy of Science USA 102, 2531-2536.
  • Joshua D. Nosanchuk, et al. (2003) Antibodies to a cell surface histone-like protein protect against Histoplasma capsulatum. Journal of Clinical Investigation 112, 1164-1175.
  • Huixing Wu, et al. (2003) Surfactant proteins A and D inhibit the growth of Gram-negative bacteria by increasing membrane permeability. Journal of Clinical Investigation 111, 1589-1602.
  • P. J. Britto, Leslie Knipling and J. Wolff. (2002) The Local Electrostatic Environment Determines Cysteine Reactivity of Tubulin. Journal of Biological Chemistry 277, 29018-29027.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision19-April-2011 RFH
    Synonyms3H-Naphthol[2,1-b]pyran-s-carboxylic Acid, 10-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)-9-methoxy-3-oxo-, Methyl Ester
    DescriptionMaleimide reagent for the detection, determination, and derivatization of thiol groups using fluorescent techniques. Superior to other thiol modifying reagents due to high fluorescent quantum yields after reaction with SH (QYSH = 0.49), extremely low fluorescence from unreacted ThioGlo® Reagent (QYR = 0.008), fast reaction times (5000 times faster than bimanes), resistance to hydrolysis (t½ ≥8.5 hr at 25°C, pH 7.0), non-interfering hydrolysis products, minimal photobleaching, large Stokes shifts (134 nm), solvachromic properties, and high sensitivity (QYSH/QYR = 60).
    FormCrystalline solid
    Intert gas (Yes/No) Packaged under inert gas
    CAS number137350-66-4
    Chemical formulaC₂₀H₁₃NO₇
    Structure formulaStructure formula
    Purity≥99% by TLC
    SolubilityDMF or DMSO (5 mg/ml)
    Storage Protect from moisture
    Protect from light
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial use, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
    Toxicity Standard Handling
    ReferencesStorey, B.T., et al. 1998. Mol. Reprod. Dev. 49, 400.
    Wright, S.K. and Viola, R.E. 1998. Anal. Biochem. 265, 8.
    Langmuir, M.E., et al. 1996. in Fluorescence Microscopy and Fluorescent Probes (Slavic, J., ed.) pp. 229-233, Plenum Press, New York.
    Citation
  • Yusheng Han, et al. (2005) Ethacrynic Acid Inhibits Multiple Steps in the NF-κB Signaling Pathway. Shock 23, 45-53.
  • John R. Pawloski, Douglas T. Hess and Jonathan S. Stamler. (2005) Impaired vasodilation by red blood cells in sickle cell disease. Proceedings of the National Academy of Science USA 102, 2531-2536.
  • Joshua D. Nosanchuk, et al. (2003) Antibodies to a cell surface histone-like protein protect against Histoplasma capsulatum. Journal of Clinical Investigation 112, 1164-1175.
  • Huixing Wu, et al. (2003) Surfactant proteins A and D inhibit the growth of Gram-negative bacteria by increasing membrane permeability. Journal of Clinical Investigation 111, 1589-1602.
  • P. J. Britto, Leslie Knipling and J. Wolff. (2002) The Local Electrostatic Environment Determines Cysteine Reactivity of Tubulin. Journal of Biological Chemistry 277, 29018-29027.
    		•