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S7700 TRAPeze® Telomerase Detection Kit

S7700
112 assays  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityKey ApplicationsDetection Methods
MaRT-PCRChromogenic
Description
Catalogue NumberS7700
Brand Family Chemicon®
Trade Name
  • TRAPeze
  • Chemicon
DescriptionTRAPeze® Telomerase Detection Kit
OverviewThe TRAPeze® Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.



Recommended Taq polymerases: must be non-proofreading, having no exonuclease activity and capable of "hot-start." Titanium Taq™, Platinum® Taq are suggested.
Alternate Names
  • TRAP Assay
Background InformationTelomeres are specific structures found at the ends of chromosomes in
eukaryotes. In human chromosomes, telomeres consist of thousands of copies of 6 base repeats (TTAGGG). It has been suggested that telomeres protect chromosome ends, because damaged chromosomes lacking telomeres undergo fusion, rearrangement and translocation. In somatic cells, telomere length is progressively shortened with each cell division both in vivo and in vitro, due to the inability of the DNA polymerase complex to replicate the very 5' endof the lagging strand .
Telomerase is a ribonucleoprotein that synthesizes and directs the telomeric
repeats onto the 3' end of existing telomeres using its RNA component as a
template. Telomerase activity has been shown to be specifically expressed in immortal cells, cancer and germ cells, where it compensates for telomere shortening during DNA replication and thus stabilizes telomere length. These observations have led to a hypothesis that telomere length may function as a “mitotic clock” to sense cell aging and eventually signal replicative senescence or programmed cell death. Therefore, expression of telomerase activity in cancer cells may be a necessary and essential step for tumor development and progression. The causal relationship between expression of telomerase and telomere length stabilization and the extension of the life span of the human cell has recently been reported.
Product Information
Detection methodChromogenic
Quality LevelMQ100
Applications
ApplicationA highly sensitive in vitro PCR based assay for detecting telomerase activity based on the TRAP assay (Telomeric Repeat Amplification Protocol).
Key Applications
  • PCR
Biological Information
Species Reactivity
  • Mammals
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size112 assays
Global Trade Item Number
Catalogue Number GTIN
S7700 04053252014062