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69025 T7•Tag® Affinity Purification Kit

Immunoaffinity purification of T7•Tag fusion proteins

T7•Tag® Affinity Purification Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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69025
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69025-3 Glass bottle 1 kit
Description
OverviewThe T7•Tag® Affinity Purification Kit is designed for rapid immunoaffinity purification of target proteins that carry the T7•Tag sequence (e.g., the 11 amino terminal aa of the T7 gene 10 protein). Purification is based on binding target proteins to T7•Tag Monoclonal Antibody that is covalently coupled to cross-linked agarose beads, washing away unbound proteins, and eluting at pH 2.2. Neutralization Buffer is included to limit protein exposure to low pH. Capacity will vary somewhat between different target proteins, but the beads are standardized to bind a minimum of 300 µg T7•Tag β-galactosidase per milliliter of settled resin. The beads can be used in either batch or column methods and can be regenerated a minimum of five times without loss of binding activity.
Catalogue Number69025
Brand Family Novagen®
References
Product Information
Components
Quality LevelMQ400
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69025-3 04055977274707

Documentation

T7•Tag® Affinity Purification Kit SDS

Title

Safety Data Sheet (SDS) 

T7•Tag® Affinity Purification Kit Certificates of Analysis

TitleLot Number
69025

Citations

Title
  • Hayakawa, H., et al. 2007. Soluble Journal of Biological Chemistry 282, 26369.
  • Chang-Sik Oh and Steven V. Beer. 2007. Plant Physiology 145, 426.
  • Sukumar, N., et al. 2007. Journal of Bacteriology 189, 3695.
  • Qiu, R., et al. 2005. Journal of Biological Chemistry 280, 16695.
  • Suzuki, T., et al. 2005. Plant and Cell Physiology 46, 1452.
  • Michael H. Kagey, Tiffany A. Melhuish and David Wotton. 2003. The Cell 113, 127.
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    User Protocols

    Title
    TB055 pET System Manual
    TB125 T7•Tag® Affinity Purification Kit