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HTS233LRTA Ready-to-Assay™ LH Glycoprotein Hormone Receptor Frozen Cells

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HTS233LRTA
1 kit  
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Overview

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This product requires a User Agreement (Label License). Please see product datasheet for further information

Key Spec Table

SpeciesHost CellsProtein Target
Human HEK293LH
Description
Catalogue NumberHTS233LRTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ LH Glycoprotein Hormone Receptor Frozen Cells
Background InformationEMD Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. EMD Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

EMD Millipore’s ChemiBrite cells co-express a GPCR along with a novel variant of clytin, a calcium-activated photoprotein, to enable sensitive luminescent detection of ligand-induced calcium flux. The ChemiBrite version of clytin contains a mutation that increases its affinity for calcium to a level that permits detection of cytosolic calcium in many cells with greater sensitivity than other mitochondrially expressed photoproteins. Luminescent calcium assays offer several advantages over fluorescent calcium assays including; lower substrate cost, increased sensitivity, and lack of interference from fluorescent compounds.

Luteinizing Hormone (LH), also known as lutropin, is produced by the pituitary gland and is critical for fertility and reproduction. In women, LH modulates ovulation. In men, LH stimulates testosterone production. Several naturally occurring mutations in the LH receptor gene have been associated with human reproductive disorders (Themmen et al.). Together with TSH, hCG and FSH, LH is a member of the glycoprotein hormone family. Each member shares a common α subunit, whereas the β subunit confers functional specificity. Upon activation, LH signals primarily through the Gαqs/adenylyl cyclase/cAMP/PKA pathway as has been shown in native granulosa cells. EMD Millipore’s cloned human LH receptor-expressing ChemiBrite cells were made by recombinant transfection of HEK293 cells with ChemiBrite clytin, the LH receptor and a promiscuous G protein to couple the receptor to the calcium signaling pathway. The cells have been cryopreserved at an optimal time post-transfection. Upon thaw, recovery, and loading, the cells are ready for luminescent, fluorescent and cAMP accumulation analysis of agonists, antagonists and modulators at the LH receptor.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Quality LevelMQ100
Applications
Key Applications
  • Calcium Flux Assays
  • cAMP Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsHEK293
Protein TargetLH
Target Sub-FamilyGlycoprotein hormone
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HTS233LRTA 04053252856723

Documentation

Ready-to-Assay™ LH Glycoprotein Hormone Receptor Frozen Cells SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
A signaling-selective, nanomolar potent allosteric low molecular weight agonist for the human luteinizing hormone receptor.
van Koppen, Chris J, et al.
Naunyn Schmiedebergs Arch. Pharmacol., 378: 503-14 (2008)  2008

Show Abstract
18551279 18551279
Mutations of gonadotropins and gonadotropin receptors: elucidating the physiology and pathophysiology of pituitary-gonadal function.
Themmen APN, and Huhtaniemi, I T
Endocr. Rev., 21: 551-83 (2000)  2000

Show Abstract
11041448 11041448
Expression of human luteinizing hormone (LH) receptor: interaction with LH and chorionic gonadotropin from human but not equine, rat, and ovine species.
Jia, X C, et al.
Mol. Endocrinol., 5: 759-68 (1991)  1991

Show Abstract
1922095 1922095

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Product Families

Categories

Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells