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540411 Puromycin, Dihydrochloride, Cell Culture-Tested - CAS 58-58-2 - Calbiochem

Puromycin, CAS 58-58-2, is a protein synthesis inhibitor that causes premature release of nascent polypeptide chains.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: protein synthesis
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
58-58-2C₂₂H₂₉N₇O₅ · 2HCl
Description
OverviewProtein synthesis inhibitor. Inhibits translation by causing premature release of nascent polypeptide chains. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
Catalogue Number540411
Brand Family Calbiochem®
References
ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.
Product Information
CAS number58-58-2
FormWhite to off-white solid
Hill FormulaC₂₂H₂₉N₇O₅ · 2HCl
Chemical formulaC₂₂H₂₉N₇O₅ · 2HCl
Quality LevelMQ100
Applications
Biological Information
Primary Targetprotein synthesis
Purity≥98% by HPLC
Physicochemical Information
ContaminantsEndotoxin: ≤10 EU/mg dry weight
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSAU7355000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage -20°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
540411 0

Documentation

Puromycin, Dihydrochloride, Cell Culture-Tested - CAS 58-58-2 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Puromycin, Dihydrochloride, Cell Culture-Tested - CAS 58-58-2 - Calbiochem Certificates of Analysis

TitleLot Number
540411

References

Reference overview
Chow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.

Brochure

Title
Antibiotics Profiler
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-April-2011 JSW
DescriptionAn aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the "A site" of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.
FormWhite to off-white solid
CAS number58-58-2
RTECSAU7355000
Chemical formulaC₂₂H₂₉N₇O₅ · 2HCl
Purity≥98% by HPLC
ContaminantsEndotoxin: ≤10 EU/mg dry weight
SolubilityH₂O (50 mg/ml) and Methanol (20 mg/ml)
Storage Protect from moisture
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Harmful
Merck USA index14, 7943
ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
de la Luna, S. and Ortín, J. 1992. Methods Enzymol. 216, 376.
Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.