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540221 Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli

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540221
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Overview

Products

Catalogue NumberPackaging Qty/Pack
540221-1MG Plastic ampoule 1 mg
Description
OverviewRecombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorolysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides. This reaction is reversible. The human enzyme is a target for therapeutic immune response intervention.
Catalogue Number540221
Brand Family Calbiochem®
SynonymsPNP
References
ReferencesFilgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun. 309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif. 27, 158.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of P<sub>i</sub> into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.
FormLiquid
FormulationIn 50 mM Tris-HCl, pH 7.6.
Quality LevelMQ100
Biological Information
PuritySingle band by SDS-PAGE
Specific Activity≥25 units/mg protein
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Global Trade Item Number
Catalogue Number GTIN
540221-1MG 04055977268751