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539785 ProteoExtract® Partial Yeast Proteome Extraction Kit

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: P-PEK Yeast Kit

539785
  
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Overview

Replacement Information
Description
OverviewThe Partial Yeast Proteome Extraction Kit (P-PEK, Yeast) is designed to extract proteins of variable solubility from yeast cells in the presence of different detergents and chaotropes. Each of the provided extraction reagents solubilizes a different subset of cellular proteins. The most soluble of the yeast proteins are released by mechanical destruction of the cells with Extraction Reagent 1, then the membrane proteins can be extracted using Extraction Reagent 3. These fractions can be analyzed on separate 2-D gel electrophoresis, and the insoluble fraction can be analyzed by one-dimensional SDS-PAGE using the SDS buffers provided. To preserve the protein profile and increase spot resolution, the kit also contains Protease Inhibitor Cocktail and the proprietary non-specific Benzonase® Nuclease (Cat. No. 71206).
Note: 1 kit is sufficient for up to 20 sample extractions.
Catalogue Number539785
Brand Family Calbiochem®
SynonymsP-PEK Yeast Kit
References
ReferencesYuan, X., et al. 2002. Electrophoresis 23, 1185.
Product Information
Form20 Extractions
Kit containsWash buffer, Extraction Reagent 1, Rehydration Reagent 2, Extraction Reagent 3A, Extraction Reagent 3B, SDS Buffer A, SDS Buffer B, glass beads, Protease Inhibitor Cocktail, Benzonase® Nuclease (Cat. No. 71206), and a user protocol.
Applications
Biological Information
Sample TypeYeast cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage FREEZER (-20°C)
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Kit containsWash buffer, Extraction Reagent 1, Rehydration Reagent 2, Extraction Reagent 3A, Extraction Reagent 3B, SDS Buffer A, SDS Buffer B, glass beads, Protease Inhibitor Cocktail, Benzonase® Nuclease (Cat. No. 71206), and a user protocol.
Specifications
Global Trade Item Number
Catalogue Number GTIN
539785 0