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539251 Protein A, Alkaline Phosphatase Conjugate

This Protein A, Alkaline Phosphatase Conjugate is validated for use in Direct Immunoassay.

Protein A, Alkaline Phosphatase Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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539251
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
539251-1MG Plastic ampoule 1 mg
Description
OverviewNative protein A conjugated to alkaline phosphatase. Can be used instead of an alkaline phosphatase-conjugated secondary antibody in many immunological assays.
Catalogue Number539251
Brand Family Calbiochem®
References
Product Information
FormLiquid
FormulationIn 50 mM Tris, 1 mM MgCl₂, 1% BSA, pH 8.0.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesDirect Immunoassay (1:5000)
Application Commentsp-Nitrophenyl phosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
PuritySingle band by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
539251-1MG 04055977268980

Documentation

Protein A, Alkaline Phosphatase Conjugate SDS

Title

Safety Data Sheet (SDS) 

Protein A, Alkaline Phosphatase Conjugate Certificates of Analysis

TitleLot Number
539251
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-August-2007 RFH
ApplicationDirect Immunoassay (1:5000)
DescriptionNative protein A from Staphylococcus aureus conjugated to alkaline phosphatase.
BackgroundProtein A is the universal "second antibody" because it binds to the Fc portions of most immunoglobulin classes. It is a versatile and economic means to isolate, detect, and purify antibodies produced by many animals. It is especially useful in immunohistochemical studies because, unlike secondary antibodies, it does not bind to cell Fc receptors, and therefore will not give high background reactions. Binding strength is as follows: strongly reactive: guinea pig, human IgG1, IgG2, and IgG4, and rabbit; weakly reactive: bovine, Human IgA, IgM, and IgE, and mouse; nonreactive: goat, horse, human IgD and IgG3, rat, and sheep.
FormLiquid
FormulationIn 50 mM Tris, 1 mM MgCl₂, 1% BSA, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
PuritySingle band by SDS-PAGE
Preservative≤0.1% sodium azide
Commentsp-Nitrophenyl phosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling