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PC459 PhosphoDetect™ Anti-VEGFR 1 (Ab-2) (pTyr¹²¹³) Rabbit pAb

This PhosphoDetect™ Anti-VEGFR 1 (Ab-2) (pTyr¹²¹³) Rabbit pAb is validated for use in ELISA, Paraffin Sections for the detection of VEGFR 1 (Ab-2) (pTyr¹²¹³).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-VEGF-R1, Anti-Flt-1, Anti-EMRK2, Anti-Vascular Endothelial Growth Factor Receptor 1

PC459
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes VEGF Receptor 1 phosphorylated at Tyr1213.
Catalogue NumberPC459
Brand Family Calbiochem®
SynonymsAnti-VEGF-R1, Anti-Flt-1, Anti-EMRK2, Anti-Vascular Endothelial Growth Factor Receptor 1
Application Data
Detection of human VEGF Receptor 1, phosphorylated at Tyr1213, by staining paraffin sections. Sample: Human breast carcinoma. Primary antibody: PhosphoDetect™ Anti-VEGF Receptor 1 (Ab-2) (pTyr1213) Rabbit pAb (Cat. No. PC459) (1:2000). Detection: DAB with methyl green counterstain.
References
ReferencesYu, Y., et al. 2001. Biochem. J. 358, 465.
Rahimi, N., et al. 2000. J. Biol. Chem. 275, 16986.
Neufeld, G., et al. 1999. FASEB J. 13, 9.
Ito, N., et al. 1998. J. Biol. Chem. 273, 23410.
Migdl, M., et al. 1998. J. Biol Chem. 273, 22272.
Soker, S. et al. 1998. Cell 92, 735.
Barleon, B., et al. 1997. J. Biol. Chem. 272, 10382.
Fournier, E., et al. 1997. Bull. Cancer 84, 397.
Kendall, R. L., et al. 1996. Biochem. Biophys. Res. Commun. 226, 324.
Devries, C., et al. 1992. Science 255, 989.
Shibuya, M., et al. 1990. Oncogene 5, 519.
Vaisman, N., et al. 1990. J. Biol. Chem. 265, 19461.
Product Information
FormLiquid
FormulationAdsorbed, undiluted serum.
Positive controlBreast carcinoma tissue
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Paraffin Sections
Application NotesELISA (see comments)
Paraffin Sections (1:2000-1:5000, heat pre-treatment required)
Application CommentsThe sequence used to generate this antibody contains a Flt-1 recognition site. This antibody can be used in conjunction with a biotinylated phosphopeptide to measure the level of Flt-1 activity by competition ELISA. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide [SDDVRpYVNAFK(C)] corresponding to amino acids 1208-1218 surrounding the Tyr¹²¹³ phosphorylation site of human vascular endothelial growth factor receptor 1
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC459 0

Documentation

PhosphoDetect™ Anti-VEGFR 1 (Ab-2) (pTyr¹²¹³) Rabbit pAb Certificates of Analysis

TitleLot Number
PC459

References

Reference overview
Yu, Y., et al. 2001. Biochem. J. 358, 465.
Rahimi, N., et al. 2000. J. Biol. Chem. 275, 16986.
Neufeld, G., et al. 1999. FASEB J. 13, 9.
Ito, N., et al. 1998. J. Biol. Chem. 273, 23410.
Migdl, M., et al. 1998. J. Biol Chem. 273, 22272.
Soker, S. et al. 1998. Cell 92, 735.
Barleon, B., et al. 1997. J. Biol. Chem. 272, 10382.
Fournier, E., et al. 1997. Bull. Cancer 84, 397.
Kendall, R. L., et al. 1996. Biochem. Biophys. Res. Commun. 226, 324.
Devries, C., et al. 1992. Science 255, 989.
Shibuya, M., et al. 1990. Oncogene 5, 519.
Vaisman, N., et al. 1990. J. Biol. Chem. 265, 19461.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-October-2007 RFH
SynonymsAnti-VEGF-R1, Anti-Flt-1, Anti-EMRK2, Anti-Vascular Endothelial Growth Factor Receptor 1
ApplicationELISA (see comments)
Paraffin Sections (1:2000-1:5000, heat pre-treatment required)
Application Data
Detection of human VEGF Receptor 1, phosphorylated at Tyr1213, by staining paraffin sections. Sample: Human breast carcinoma. Primary antibody: PhosphoDetect™ Anti-VEGF Receptor 1 (Ab-2) (pTyr1213) Rabbit pAb (Cat. No. PC459) (1:2000). Detection: DAB with methyl green counterstain.
DescriptionRabbit polyclonal antibody supplied as undiluted serum adsorbed against non-phosphorylated immunogen peptide. Recognizes the ~180 kDa VEGFR-1 protein.
BackgroundThe vascular endothelial growth factor receptor-1 (VEGFR-1, approximately 180 kDa) protein is a member of a subfamily of receptor-tyrosine kinases characterized by the presence of seven immunoglobulin-like domains in the extracellular portion of the molecules and a split tyrosine-kinase domain intracellularly. Also known as Flt-1, VEGFR-1 is expressed primarily in endothelial cells but has also been demonstrated in various tumor cells, trophoblast cells, monocytes, and mesangial cells. The receptor is a glycoprotein but glycosylation is not required for activity. Following ligand binding VEGFR-1 undergoes autophosphorylation with the major sites being Tyr-1213 and Tyr-1333. Phosphorylation of Tyr-1213 in turn allows the binding of Nck, SHP-2 and P13K to the VEGFR-1 protein and subsequent signal transduction cascades initiated. Interestingly, ligand binding to VEGFR-1 overexpressed in cell types other than endothelial cells fails to initiate a proliferative response suggesting a unique signal cascade in the endothelial cell.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide [SDDVRpYVNAFK(C)] corresponding to amino acids 1208-1218 surrounding the Tyr¹²¹³ phosphorylation site of human vascular endothelial growth factor receptor 1
IsotypeIgG
Specieshuman
Positive controlBreast carcinoma tissue
FormLiquid
FormulationAdsorbed, undiluted serum.
PreservativeNone
CommentsThe sequence used to generate this antibody contains a Flt-1 recognition site. This antibody can be used in conjunction with a biotinylated phosphopeptide to measure the level of Flt-1 activity by competition ELISA. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesYu, Y., et al. 2001. Biochem. J. 358, 465.
Rahimi, N., et al. 2000. J. Biol. Chem. 275, 16986.
Neufeld, G., et al. 1999. FASEB J. 13, 9.
Ito, N., et al. 1998. J. Biol. Chem. 273, 23410.
Migdl, M., et al. 1998. J. Biol Chem. 273, 22272.
Soker, S. et al. 1998. Cell 92, 735.
Barleon, B., et al. 1997. J. Biol. Chem. 272, 10382.
Fournier, E., et al. 1997. Bull. Cancer 84, 397.
Kendall, R. L., et al. 1996. Biochem. Biophys. Res. Commun. 226, 324.
Devries, C., et al. 1992. Science 255, 989.
Shibuya, M., et al. 1990. Oncogene 5, 519.
Vaisman, N., et al. 1990. J. Biol. Chem. 265, 19461.