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569372 PhosphoDetect™ Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6)

This PhosphoDetect™ Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of Src (pTyr⁴¹⁸).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~60 kDa Src protein phosphorylated at Tyr418 in chick embryo fibroblasts expressing human wild-type Src.
Catalogue Number569372
Brand Family Calbiochem®
Application Data
Detection of mouse Src, phosphorylated on Tyr423, by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells cultured under serum-free conditions, left untreated (lanes 1), or treated with H2O2 (lane 2). Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (0.1 µg/ml) Detection: chemiluminescence.

Detection of human Src, phosphorylated on Tyr418, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (left lane) or treated with vanadate (right lane). Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (0.1 µg/ml). Detection: chemiluminescence.

Detection of human Src, phosphorylated on Tyr418, by immunocytochemistry. Sample: A431 cells treated with EGF for 15 min. Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (1 µg/ml). Detection: fluorescence.
References
ReferencesMartin, G.S. 2001. Nat. Rev. Mol. Cell Biol. 2, 467.
Reissig, D., et al. 2001. J. Cancer Res. Clin. Oncol. 127, 226.
Bjorge, J.D., et al. 2000. Oncogene 19, 5620.
Irby, R.B. and Yeatman, T.J. 2001. Oncogene 19, 5636.
Miermont, A.M., et al. 2000. J. Immunol. Methods 246, 203.
Product Information
FormLyophilized
FormulationLyophilized from 2X PBS, PEG, sucrose.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1 µg/ml, immunofluorescence)
Application CommentsDoes not cross-react with non-phosphorylated Src, or with unrelated phosphorylation sites. The sequence of the immunogen is conserved in human (Tyr418), mouse (Tyr423), rat (Tyr418), chicken (Tyr416), and c-Src (Tyr416). Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the pTyr⁴¹⁸ phosphorylation site of human Src
ImmunogenHuman
Clone9A6
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute in 1 ml sterile dH₂O (15 min, room temperature). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
569372 0

Documentation

PhosphoDetect™ Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6) SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-Src (pTyr⁴¹⁸) Mouse mAb (9A6) Certificates of Analysis

TitleLot Number
569372

References

Reference overview
Martin, G.S. 2001. Nat. Rev. Mol. Cell Biol. 2, 467.
Reissig, D., et al. 2001. J. Cancer Res. Clin. Oncol. 127, 226.
Bjorge, J.D., et al. 2000. Oncogene 19, 5620.
Irby, R.B. and Yeatman, T.J. 2001. Oncogene 19, 5636.
Miermont, A.M., et al. 2000. J. Immunol. Methods 246, 203.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-August-2007 RFH
ApplicationELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1 µg/ml, immunofluorescence)
Application Data
Detection of mouse Src, phosphorylated on Tyr423, by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells cultured under serum-free conditions, left untreated (lanes 1), or treated with H2O2 (lane 2). Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (0.1 µg/ml) Detection: chemiluminescence.

Detection of human Src, phosphorylated on Tyr418, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (left lane) or treated with vanadate (right lane). Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (0.1 µg/ml). Detection: chemiluminescence.

Detection of human Src, phosphorylated on Tyr418, by immunocytochemistry. Sample: A431 cells treated with EGF for 15 min. Primary antibody: PhosphoDetect™ Anti-Src (pTyr418) Mouse mAb (9A6) (Cat. No. 569372) (1 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~60 kDa Src protein phosphorylated at Tyr418 (human, rat), Tyr423 (mouse), and Tyr416 (chicken, v-Src).
BackgroundSrc belongs to a family of 9 tyrosine kinases which regulate cellular responses to extracellular stimuli. Src is a non-receptor protein tyrosine kinase that transduces signals that are involved in the control of a variety of cellular processes such as proliferation, differentiation, motility, and adhesion. Src is normally maintained in an inactive state, but can be activated transiently during cellular events such as mitosis, or constitutively by abnormal events such as mutation (i.e. v-Src and some human cancers). Src becomes activated upon dephosphorylation of Tyr527 which leads to a conformational change of the Src molecule. This allows binding to other proteins via the SH2 and SH3 domain. Activated Src phosphorylates a variety of substrates involved in the mitogenic signaling pathway, cytoskeletal organization, cell-substrate adhesion, cell-cell adhesion, cell-cell communication and RNA processing.
HostMouse
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the pTyr⁴¹⁸ phosphorylation site of human Src
Clone9A6
IsotypeIgG₁
Specieshuman, mouse, rat
FormLyophilized
FormulationLyophilized from 2X PBS, PEG, sucrose.
Preservative≤0.1% sodium azide
CommentsDoes not cross-react with non-phosphorylated Src, or with unrelated phosphorylation sites. The sequence of the immunogen is conserved in human (Tyr418), mouse (Tyr423), rat (Tyr418), chicken (Tyr416), and c-Src (Tyr416). Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute in 1 ml sterile dH₂O (15 min, room temperature). Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesMartin, G.S. 2001. Nat. Rev. Mol. Cell Biol. 2, 467.
Reissig, D., et al. 2001. J. Cancer Res. Clin. Oncol. 127, 226.
Bjorge, J.D., et al. 2000. Oncogene 19, 5620.
Irby, R.B. and Yeatman, T.J. 2001. Oncogene 19, 5636.
Miermont, A.M., et al. 2000. J. Immunol. Methods 246, 203.