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NB13 PhosphoDetect™ Anti-Ca2+/Calmodulin Kinase II α-Subunit (pThr²⁸⁶) (Ab-2) Mouse mAb (22B1)

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NB13
  
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Overview

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~50 kDa α subunit of CaM kinase II phosphorylated at Thr286. Does not cross-react with unphosphorylated α subunit of CaM kinase II or with the β subunit in either phosphorylation state.
Catalogue NumberNB13
Brand Family Calbiochem®
Application Data
Detection of rat Ca2+/calmodulin kinase II α-subunit, phosphorylated on Thr286, by immunoblotting. Samples: Whole tissue extract from rat brain, unphosphorylated (lane 1) and phosphorylated (lane 2). Primary antibody: PhosphoDetect™ Anti-Ca2+/Calmodulin Kinase II α-Subunit (pThr286) (Ab-2) Mouse mAb (22B1) (Cat. No. NB13) (1:2000). Detection: chemiluminescence.
References
ReferencesMayford, M., et al. 1996. Science 274, 1678.
Lisman, J. 1994. Trends Neurosci. 17, 406.
Ochiishi, T., et al. 1994. Brain Res. 659, 179.
Hanson, P.I. and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.
Silva, A.J., et al. 1992. Science 257, 201.
Erondu, N.E. and Kennedy, M.B. 1985. J. Neurosci. 5, 3270.
Product Information
FormLiquid
FormulationIn PBS.
Preservative≤0.1% sodium azide
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunofluorescence
Application NotesELISA (see comments)
Immunoblotting (1:2000)
Immunofluorescence (1:100)
Application CommentsDoes not cross-react with the non-phosphorylated α subunit of CaM kinase II or the 60 kDa β subunit in either phosphorylation state. In rat hippocampal cells, this antibody labels cell somas and neuropil, while nuclei are only lightly stained by immunofluorescence. This antibody has been reported to work for ELISA. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide (MHRQEpTVDCLKKFN) corresponding to amino acids 281-294 surrounding the Thr²⁸⁶ phosphorylation site of mouse and rat CaM kinase II α subunit
ImmunogenMouse and Rat
Clone22B1
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
NB13 0