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17-126 PTP Assay Kit 2

PTP Assay Kit 2 can be used to detect PTP-1B activity by either dephosphorylation of the phosphopeptide (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) or hydrolysis of pNPP.

PTP Assay Kit 2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Phosphatase Assay
17-126
1 kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsAnalytes AvailableDetection Methods
PAPTP Colorimetric
Description
Catalogue Number17-126
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPTP Assay Kit 2
References
Product Information
Components
  • Tyrosine Phosphopeptide (TSTEPQpYQPGENL) (Cat.# 12-218)
  • Malachite Green Solution A (Cat.# 20-105)
  • Malachite Green Additive (Cat.# 20-104)
  • Phosphate Standard (Cat.# 20-103)
  • pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
  • pNPP Tyr Assay Buffer (Cat.# 20-180)
  • 96-well microtiter plate
Detection methodColorimetric
Quality LevelMQ100
Applications
ApplicationPTP Assay Kit 2 can be used to detect PTP-1B activity by either dephosphorylation of the phosphopeptide (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) or hydrolysis of pNPP.
Key Applications
  • Phosphatase Assay
Biological Information
Analytes Available
  • PTP
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
Gene Symbol
  • PTPN1
  • PTP-1B
  • PTP1B
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P18031 # May play an important role in CKII- and p60c-src-induced signal transduction cascades (By similarity).
SIZE: 435 amino acids; 49967 Da
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.
PTM: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
SIMILARITY: SwissProt: P18031 ## Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. & Contains 1 tyrosine-protein phosphatase domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceThe components in this kit were used to detect PTP-1B (Catalog # 14-109) activity by either dephosphorylation of the phosphopeptide (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) or hydrolysis of pNPP.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
17-126 04053252407222

Documentation

PTP Assay Kit 2 SDS

Title

Safety Data Sheet (SDS) 

PTP Assay Kit 2 Certificates of Analysis

TitleLot Number
PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) - 2459578 2459578
PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 3125647
PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2970636
PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2927702
PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 3030840
PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2905533
PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2896282
PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2958930
PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) - 2197520 2197520
PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) - 2279221 2279221
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References

Reference overviewApplicationPub Med ID
The serine phosphatases PP1 and PP2A associate with and activate the actin-binding protein cofilin in human T lymphocytes
Ambach, A., et al
Eur J Immunol, 30:3422-31 (2000)  2000

Phosphatase Assay11093160 11093160
Phosphorylated synthetic peptides as tools for studying protein phosphatases
Pinna, L. A. and Donella-Deana, A.
Biochim Biophys Acta, 1222:415-31 (1994)  1994

Phosphatase Assay8038211 8038211
Inorganic and organic phosphate measurements in the nanomolar range.
Van Veldhoven, P P and Mannaerts, G P
Anal. Biochem., 161: 45-8 (1987)  1987

Show Abstract 		Phosphatase Assay3578786 3578786

FAQ

QuestionAnswer
I still have material left to run more assays, but I need another plate. Where can I find a comparable plate?To get a comparable plate, you can purchase a Costar Brand 96-Well Half Area Plate (for Tissue Culture Section).