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539493 PP1, α-Isoform, Rabbit Muscle, Recombinant, E. coli

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Overview

Description
Overview

This product has been discontinued.



Recombinant, rabbit, Mn2+-dependent catalytic subunit of the α-isoform of protein phosphatase 1 expressed in E. coli. Hydrolyzes the phosphate groups on serine and threonine residues and has also been known to hydrolyze phosphates on tyrosine residues. Assists in tobacco mosaic virus-mediated programmed cell death.

Catalogue Number539493
Brand Family Calbiochem®
SynonymsProtein Phosphatase 1, α-Isoform, Phosphorylase Phosphatase
References
ReferencesDunigan, D.D., and Madlener, J.C. 1995. Virology 207, 460.
Zhang, Z., et al. 1993. Mol. Cell. Biochem. 127, 113.
Zhang, A.J., et al. 1992. J. Biol. Chem. 267, 1484.
Product Information
Activity≥500 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per minute at 30°C, pH 7.0.
FormLiquid
FormulationIn 200 mM NaCl, 50 mM HEPES, 2.5 mM DTT, 1 mM MnCl₂, 0.1 mM EGTA, 50% glycerol, 0.025% TWEEN®-20 Detergent, pH 7.0.
Biological Information
Specific Activity13,500 units/mg
Physicochemical Information
ContaminantsProtease: none detected
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°c).
Global Trade Item Number
Catalogue Number GTIN
539493 0