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521200 PDGF, Human Platelets

PDGF, Human Platelets MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: nhPDGF

521200
  
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Overview

Replacement Information

Key Spec Table

Purity
≥97% by SDS-PAGE
Description
Overview

This product has been discontinued.





Native platelet-derived growth factor from human platelets. Consists of approximately 70% PDGF-AB and 30% PDGF-BB. PDGF action is mediated by cell surface α- and β-receptors. M.W. 28,000-32,000.
Catalogue Number521200
Brand Family Calbiochem®
SynonymsnhPDGF
References
ReferencesAbboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
Soma, Y., et al. 1994. Exp. Cell Res. 212, 274.
Hammacher, A., et al. 1988. J. Biol. Chem. 263, 16493.
Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
Product Information
FormLyophilized
FormulationLyophilized from sterile-filtered 25% acetonitrile, 0.1% TFA.
Applications
Biological Information
Biological activityED₅₀ = 1-3 ng/ml as measured in a mitogenic assay using quiescent NR 6-3T3 fibroblasts
Purity≥97% by SDS-PAGE
SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Physicochemical Information
ContaminantsEndotoxin: ≤1 EU/µg PDGF
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
521200 0

Documentation

PDGF, Human Platelets Certificates of Analysis

TitleLot Number
521200

References

Reference overview
Abboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
Soma, Y., et al. 1994. Exp. Cell Res. 212, 274.
Hammacher, A., et al. 1988. J. Biol. Chem. 263, 16493.
Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-May-2008 RFH
SynonymsnhPDGF
DescriptionNative platelet-derived growth factor from human platelets. Consists of approximately 70% PDGF-AB and may contain about 30% PDGF-BB. PDGF action is mediated by cell surface α- and β-receptors.
FormLyophilized
FormulationLyophilized from sterile-filtered 25% acetonitrile, 0.1% TFA.
SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: ≤1 EU/µg PDGF
Biological activityED₅₀ = 1-3 ng/ml as measured in a mitogenic assay using quiescent NR 6-3T3 fibroblasts
SolubilityReconstitute to a concentration ≥10 µg/ml with sterile 4 mM HCl containing at least 0.1% HSA or BSA.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Toxicity Standard Handling
Merck USA index14, 7525
ReferencesAbboud, H.E., et al. 1994. J. Cell Physiol. 158, 140.
Soma, Y., et al. 1994. Exp. Cell Res. 212, 274.
Hammacher, A., et al. 1988. J. Biol. Chem. 263, 16493.
Raines, E.W., et al. 1985. Methods Enzymol. 109, 749.