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482722 Nitric Oxide Synthase, Constitutive, Rat Neuronal, Recombinant, High Purity

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: nNOS, Rat, Neuronal Nitric Oxide Synthase

482722
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.





Recombinant, rat neuronal NOS expressed in S. frugiperda insect cells using a baculovirus expression system.
Catalogue Number482722
Brand Family Calbiochem®
SynonymsnNOS, Rat, Neuronal Nitric Oxide Synthase
References
ReferencesAbu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32047.
Abu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32318.
Richards, M.K., and Marletta, M.A. 1994. Biochemistry 33, 14723.
Sheta, E.A., et al. 1994. J. Biol. Chem. 269, 15147.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will produce 1 nmol of nitric oxide per min in the presence of calmodulin at 37°C, pH 7.5.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 10 µM tetrahydro-L-biopterin, 20% glycerol, pH 7.4.
Applications
Biological Information
Purity≥95% by SDS PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 20/21/22-36/37/38

Harmful by inhalation, in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Harmful
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
482722 0

Documentation

Nitric Oxide Synthase, Constitutive, Rat Neuronal, Recombinant, High Purity Certificates of Analysis

TitleLot Number
482722

References

Reference overview
Abu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32047.
Abu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32318.
Richards, M.K., and Marletta, M.A. 1994. Biochemistry 33, 14723.
Sheta, E.A., et al. 1994. J. Biol. Chem. 269, 15147.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-August-2008 RFH
SynonymsnNOS, Rat, Neuronal Nitric Oxide Synthase
DescriptionRecombinant, rat neuronal NOS expressed in S. frugiperda insect cells using a baculovirus expression system. Useful for studies involving NOS regulation, inhibitor screening, and kinetic analysis.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 10 µM tetrahydro-L-biopterin, 20% glycerol, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Combine the following at 37°C: 50 mM        HEPES, pH 7.4 12 µM        BH4 1 mM         CaCl2 20 µg/ml   Calmodulin 100 µM     NADPH 50 µM      Arginine 120 µM     DTT 3 µM        Oxyhemoglobin Measure spectrophotometrically at 401 nm.
Purity≥95% by SDS PAGE
Unit definitionOne unit is defined as the amount of enzyme that will produce 1 nmol of nitric oxide per min in the presence of calmodulin at 37°C, pH 7.5.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Harmful
ReferencesAbu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32047.
Abu-Soud, H.M., et al. 1994. J. Biol. Chem. 269, 32318.
Richards, M.K., and Marletta, M.A. 1994. Biochemistry 33, 14723.
Sheta, E.A., et al. 1994. J. Biol. Chem. 269, 15147.