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FCABS326PE Milli-Mark® Anti-acetyl-Histone H4 Antibody-PE

Milli-Mark® Anti-acetyl-Histone H4 Antibody-PE is a highly specific rabbit polyclonal antibody, that targets Histone H4 & has been tested in Flow Cytometry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins, Histones Gene Symbol: HHF1, HHF2 UniProt Number: P69152
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FCABS326PE
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCRbPhycoerythrinPolyclonal Antibody
Description
Catalogue NumberFCABS326PE
Trade Name
  • Milli-Mark
DescriptionMilli-Mark® Anti-acetyl-Histone H4 Antibody-PE
Alternate Names
  • Histone H4
Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
References
Product Information
FormatPhycoerythrin
Control
  • HeLa cells
PresentationPurified rabbit polyclonal IgG conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationMilli-Mark® Anti-acetyl-Histone H4 Antibody-PE is a highly specific rabbit polyclonal antibody, that targets Histone H4 & has been tested in Flow Cytometry.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenTetrahymena histone H4 acetylated on lysines 5, 8, 12, and 16.
HostRabbit
SpecificityRecognizes acetylated histone H4. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May crossreact with other acetylated proteins.
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element [provided by RefSeq].
Gene Symbol
  • HHF1
  • HHF2
Modifications
  • Acetylation
  • Acetylation
  • Acetylation
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

SIZE: 103 amino acids; 11367 Da

SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

SUBCELLULAR LOCATION: Nucleus.

PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.

SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
Molecular Weight11 kDa Calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by flow cytometry using HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
FCABS326PE 04053252820663

Documentation

Milli-Mark® Anti-acetyl-Histone H4 Antibody-PE SDS

Title

Safety Data Sheet (SDS) 

Milli-Mark® Anti-acetyl-Histone H4 Antibody-PE Certificates of Analysis

TitleLot Number
Milli-Mark Anti-acetyl-Histone H4-PE - Q2120878 Q2120878
Milli-Mark™ Anti-acetyl-Histone H4-PE 2480971
Milli-Mark™ Anti-acetyl-Histone H4-PE - 3601743 3601743
Milli-Mark™ Anti-acetyl-Histone H4-PE - 3698190 3698190
Milli-Mark™ Anti-acetyl-Histone H4-PE - 3928036 3928036
Milli-Mark™ Anti-acetyl-Histone H4-PE - 4122166 4122166
Milli-Mark™ Anti-acetyl-Histone H4-PE -2594451 2594451
Milli-Mark™ Anti-acetyl-Histone H4-PE -2608860 2608860
Milli-Mark™ Anti-acetyl-Histone H4-PE -2649274 2649274
Milli-Mark™ Anti-acetyl-Histone H4-PE -2686134 2686134
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