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444256 MMP Substrate III, Fluorogenic - Calbiochem

A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination.

MMP Substrate III, Fluorogenic - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: DABCYL-GABA-PQGL-E(EDANS)-AK-NH₂, TNO211

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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
444256-500UG Plastic ampoule 500 μg
Description
OverviewA specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity [kcat/Km = 619,000 M-1s-1 for MMP-2, 206,000 M-1s-1 for MMP-9, 40,000 M-1s-1 for MMP-3, and 21,000 M-1s-1 for MMP-1; at 37°C, pH 7.6 ]. Exhibits a high degree of sensitivity that is not affected by optical disturbances in biological media. Also useful for MMP activity measurements in synovial fluid and culture medium.
Catalogue Number444256
Brand Family Calbiochem®
SynonymsDABCYL-GABA-PQGL-E(EDANS)-AK-NH₂, TNO211
References
ReferencesBeekman, B., et al. 1996. FEBS Lett 390, 221.
Product Information
ATP CompetitiveN
FormRed lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₆₃H₈₈N₁₆O₁₄S
Chemical formulaC₆₃H₈₈N₁₆O₁₄S
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetDetection of MMP activity
Purity≥97% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceDABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH₂
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
444256-500UG 04055977186543

Documentation

MMP Substrate III, Fluorogenic - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

MMP Substrate III, Fluorogenic - Calbiochem Certificates of Analysis

TitleLot Number
444256

References

Reference overview
Beekman, B., et al. 1996. FEBS Lett 390, 221.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-August-2008 RFH
SynonymsDABCYL-GABA-PQGL-E(EDANS)-AK-NH₂, TNO211
DescriptionA fluorescence energy transfer (FRET) peptide substrate with MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity (kcat/Km = 619,000 M-1s-1 for MMP-2, 206,000 M-1s-1 for MMP-9, 40,000 M-1s-1 for MMP-3, 21,000 M-1s-1 for MMP-1; pH = 7.6 at 37°C). Exhibits a high degree of sensitivity, which is not affected by optical disturbances in biological media. Excitation max.: ~340 nm; emission max.: ~485 nm.
FormRed lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₆₃H₈₈N₁₆O₁₄S
Peptide SequenceDABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH₂
Purity≥97% by HPLC
SolubilityDMSO and Tris buffered saline (pH 7.6)
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesBeekman, B., et al. 1996. FEBS Lett 390, 221.