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420411 Kanamycin Sulfate, Streptomyces kanamyceticus, Cell Culture-Tested - CAS 25389-94-0 - Calbiochem

An aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 30S ribosome, causing misreading of the genetic code.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: protein biosynthesis that acts on the 30S ribosome
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
25389-94-0C₁₈H₃₆N₄O₁₁ · H₂SO₄
Description
OverviewAn aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 30S ribosome, causing misreading of the genetic code. May cause renal damage and ototoxicity.
Contains >98% kanamycin A. Potency: ≥750 µg/mg
Recommended working concentration: 100 µg/ml.
Catalogue Number420411
Brand Family Calbiochem®
References
ReferencesLech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1 (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.
Cardon, G.H., et al. 1993. Plant J. 3, 773.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York. p.1.6, A.6.
Korzybyski, T., et al. 1978. In Antibiotics, Origin, Nature, and Properties 1, 690.
Stiffler, P.W., et al. 1974. Antimicrob. Agents Chemother. 6, 516.
Umezawa, S., et al. 1968. J. Antibiot. 21, 367.
Product Information
CAS number25389-94-0
FormWhite to off-white solid
Hill FormulaC₁₈H₃₆N₄O₁₁ · H₂SO₄
Chemical formulaC₁₈H₃₆N₄O₁₁ · H₂SO₄
Quality LevelMQ100
Applications
Biological Information
Primary Targetprotein biosynthesis that acts on the 30S ribosome
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSNZ3225030
Safety Information
R PhraseR: 36/37/38-42/43-60-61

Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
May impair fertility.
May cause harm to the unborn child.
S PhraseS: 26-36/37/39-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Carcinogenic / Teratogenic
Storage +15°C to +30°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
420411 0

Documentation

Kanamycin Sulfate, Streptomyces kanamyceticus, Cell Culture-Tested - CAS 25389-94-0 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Kanamycin Sulfate, Streptomyces kanamyceticus, Cell Culture-Tested - CAS 25389-94-0 - Calbiochem Certificates of Analysis

TitleLot Number
420411

References

Reference overview
Lech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1 (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.
Cardon, G.H., et al. 1993. Plant J. 3, 773.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York. p.1.6, A.6.
Korzybyski, T., et al. 1978. In Antibiotics, Origin, Nature, and Properties 1, 690.
Stiffler, P.W., et al. 1974. Antimicrob. Agents Chemother. 6, 516.
Umezawa, S., et al. 1968. J. Antibiot. 21, 367.

Brochure

Title
Antibiotics Profiler
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-September-2010 JSW
DescriptionAn aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 70S ribosome, causing misreading of the genetic code. May cause renal damage and ototoxicity. Useful for the selection of kanamycin resistant plasmids in E. coli. Tested and found to be negative for cytotoxicity against HeLa cells. Contains more than 98% kanamycin A.
FormWhite to off-white solid
CAS number25389-94-0
RTECSNZ3225030
Chemical formulaC₁₈H₃₆N₄O₁₁ · H₂SO₄
SolubilityH₂O (10 mg/ml)
Storage +15°C to +30°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.
Toxicity Carcinogenic / Teratogenic
Merck USA index14, 5281
ReferencesLech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1 (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.
Cardon, G.H., et al. 1993. Plant J. 3, 773.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York. p.1.6, A.6.
Korzybyski, T., et al. 1978. In Antibiotics, Origin, Nature, and Properties 1, 690.
Stiffler, P.W., et al. 1974. Antimicrob. Agents Chemother. 6, 516.
Umezawa, S., et al. 1968. J. Antibiot. 21, 367.