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CBA013 InnoCyte™ ECM Cell Adhesion Assay, Collagen Type IV

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CBA013
  
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Overview

Key Spec Table

Detection Methods
Fluorometric
Description
OverviewA convenient assay for the determination of the relative attachment of adherent cell lines to collagen type IV. Cells are seeded onto the collagen type IV coated plates followed by determination of relative cell attachment using a fluorescent dye.
Catalogue NumberCBA013
Brand Family Calbiochem®
Materials Required but Not Delivered• Pipettors with disposable tips
• Fluorescence reader capable of measuring fluorescence in 96-well plates with a filter set of excitation wavelength ~485 nm and emission wavelength ~520 nm
• PBS, preferably containing calcium/magnesium (Dulbecco's PBS) for washing
References
ReferencesCaroll, D.K., et al. 2006. Nat. Cell Biol. 8, 551.
Hynes, R.O., et al. 1999. Proc. Natl. Acad. Sci. USA96, 2588.
Ohashi, T., et al. 1999. Proc. Natl. Acad. Sci USA 96, 2153.
Grande, J.P., et al. 1997. J. Lab. Clin. Med. 130, 476.
Nelson, P.R., et al. 1997. Vasc. Surg. 26, 104.
Horowitz, A.F., et al. 1996. Trends Cell Biol. 6, 460.
Hynes, R.O., et al. 1990. Fibronectins, Springer-Verlag, New York.
Preissner, K.T., et al. 1990. J. Biol. Chem. 265, 18490.
Product Information
Detection methodFluorometric
Form72 Tests
Format96-well plate
Kit containsCoated 96-Well Plate, Fluorescent Dye, D-PBS, and a user protocol.
Applications
Application ReferencesCaroll, D.K., et al. 2006. Nat. Cell Biol. 8, 551.
Biological Information
Assay time2.5 h
Sample TypeAdherent cultured cells
Physicochemical Information
Emission max.
Excitation max.
Product Usage Statements
Intended useThe Innocyte™ Cell Adhesion Microplate Assays are designed for the determination of the relative attachment of adherent cell lines to extracellular matrix proteins such as Human Fibronectin (Cat. No. CBA011), Human Vitronectin (Cat. No. CBA012), and Human Collagen IV (Cat. No. CBA013). Cells are seeded onto a coated substrate, which is followed by the determination of relative cell attachment using a fluorescent dye.
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Storage ConditionsUpon arrival store the Calcein-AM at -20°C and the remaining components at 4°C.
Protect from Light Protect from light
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Supplemental Information
Kit containsCoated 96-Well Plate, Fluorescent Dye, D-PBS, and a user protocol.
Global Trade Item Number
Catalogue Number GTIN
CBA013 0