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IEVH85R Immobilon®-E PVDF Membrane

The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.

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IEVH85R
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Overview

Replacement Information
Description
Catalogue NumberIEVH85R
Trade Name
  • Immobilon
DescriptionImmobilon®-E PVDF Membrane
OverviewImmobilon-E is the first PVDF membrane for Western blotting that can be wetted with with aqueous transfer buffer. This eliminates the alcohol pre-wet step required with other PVDF membranes.
Background InformationThis 8.5cm x 10m roll offers 1-cut convenience for standard mini-blots (7 x 8.5cm) and midi-blots (8.5 x 13.5cm). It works with the optional Immobilon NOW Dispenser (catalog number IMDISP).

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most Western blotting applications, especially proteins greater than 20 kDa
- Compatible with chemiluminescent and chromogenic detection

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes.

Features & Benefits:
- High protein binding provides stronger signal
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be stripped and reprobed multiple times
References
Product Information
Detection methodChemiluminescence Chromogenic Radioactive
Compatible Stains
  • Coomassie brilliant blue
  • Amido black
  • India ink
  • Ponceau-S red
  • Colloidal gold
  • Transillumination
  • Sypro® ruby
Filter CodeIEVH
Filter ColorWhite
Quality LevelMQ400
Applications
ApplicationThe first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
Key Applications
  • Western Blotting
  • Immunoblotting (Western)
Biological Information
Primary Binding MechanismsElectrostatic, hydrophobic
BSA AdsorptionNot Applicable
WettabilityEasily wetted with water
Physicochemical Information
Pore Size0.45 µm
Dimensions
Filter SurfacePlain
Filter Dimensions8.5 cm x 10 m
Materials Information
Chemistry
  • Polyvinylidene Fluoride (PVDF)
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Packaging Information
Material Size1
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IEVH85R 04054839569098

Related Products & Applications

Product Families

Immobilon® Membranes, Sandwiches and Blotting Filter Paper

Immobilon® PVDF membranes are the ideal transfer membranes for protein blotting applications.
Learn More >>