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NWKITIE Immobilon®-E NOW Bundle

The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.

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Overview

Replacement Information
Description
Catalogue NumberNWKITIE
Trade Name
  • Immobilon
DescriptionImmobilon®-E NOW Bundle
OverviewImmobilon-E is the first PVDF membrane for Western blotting that can be wetted with with aqueous transfer buffer. This eliminates the alcohol pre-wet step required with other PVDF membranes.
Background InformationThis bundle includes the Immobilon NOW Dispenser and two 8.5cm x 10m rolls of Immobilon-E transfer membrane. The Immobilon NOW system offers 1-cut convenience for standard mini-blots (7 x 8.5cm) and midi-blots (8.5 x 13.5cm).

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most Western blotting applications, especially proteins greater than 20 kDa
- Compatible with chemiluminescent and chromogenic detection
References
Product Information
Detection methodChemiluminescence Chromogenic Radioactive Colorimetric
Compatible Stains
  • Coomassie brilliant blue
  • Amido black
  • India ink
  • Ponceau-S red
  • Colloidal gold
  • Transillumination
  • Sypro® ruby
Filter CodeIEVH
Filter ColorWhite
Applications
ApplicationThe first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
Key Applications
  • Western Blotting
  • Immunoblotting (Western)
  • Dot Blot
Biological Information
Primary Binding MechanismsElectrostatic, hydrophobic
BSA AdsorptionNot Applicable
WettabilityEasily wetted with water
Physicochemical Information
Pore Size0.45 µm
Dimensions
Filter SurfacePlain
Filter Dimensions8.5 cm x 10 m
Materials Information
Chemistry
  • Polyvinylidene Fluoride (PVDF)
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Packaging Information
Material Size1
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NWKITIE 04054839625473

Documentation

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