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FC010-100MG Human Plasma Fibronectin Purified Protein

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FC010-100MG
100 mg  
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Overview

Key Spec Table

Key Applications
CULT
Description
Catalogue NumberFC010-100MG
ReplacesFC010-250MG
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionHuman Plasma Fibronectin Purified Protein
OverviewHuman fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.
Product Information
PresentationLyophilized from 50 mM tris-buffered saline (TBS), pH 7.5, containing no preservatives.
Quality LevelMQ100
Applications
Key Applications
  • Cell Culture
Application NotesImmunochemical standard

Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts

Suggested Procedure for Coating Cell Cultureware

1. Determine the amount of hFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (mg/mL) of hFN. Recommended amount is 2-10 mg/cm2.

2. Reconstitute the entire vial* of hFN to 1mg/mL a sterile balanced salt solution at 37ºC. Add the lyophilized hFN slowly with constant stirring, and avoid excessive foaming. Break up any clumps that might form against the side of the container with a glass rod or spatula. Sterile filter the hFN solution to remove any remaining insoluble material. Store the reconstituted material at 2º-8° C for up to 6 months. For working dilutions, dilute the material to the desired concentration with physiological buffer. For determining final protein concentrations by OD280, the Extinction coefficient = 1.3 [(mg/mL)xcm]-1).

3. Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

4. Introduce the proper CO2 atmosphere, if required.

5. Add the calculated amount of hFN to each culture vessel and store the remaining hFN at 2º-8ºC as indicated below.

6. Allow hFN to adsorb to the surface of the vessel for 5-20 minutes.

7. Remove residual balanced salt solution before proceeding with standard cell culture procedures.



*Note: hFN is lyophilized from buffered saline and the weight of the material is not only of the protein itself, but includes the protein + salts. The material may not be homogeneous with regard to protein/salt ratio.
Biological Information
PurityApproximately 95%, as determined by SDS-PAGE. A double band of 220 kDa is present under reduced conditions. HFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose.
SourceHuman donor plasma.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
Gene Symbol
  • FN1
  • DKFZp686O13149
  • DKFZp686H0342
  • DKFZp686I1370
  • MSF
  • FN
  • CIG
  • DKFZp686F10164
  • LETS
  • FINC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
SIZE: 2386 amino acids; 262607 Da
SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
PTM: Sulfated.
SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
Stem Cell Type
  • Human Embryonic Stem Cells
  • Mesenchymal Stem Cells
  • Neural Stem Cells
  • Hematopoietic Stem Cells
  • Epithelial Cells
  • Pancreatic Stem Cells
  • Induced Pluripotent Stem Cells
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months from date of receipt. Store the reconstituted material at 2º-8° C for up to 6 months.
Packaging Information
Material Size100 mg
Global Trade Item Number
Catalogue Number GTIN
FC010-100MG 04053252474866