Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

12-500 HeLa + EGF Stimulated Cell Lysate in Mg2+ Lysis Buffer

View Products on Sigmaaldrich.com
12-500
200 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
WB
Description
Catalogue Number12-500
Brand Family Upstate
Trade Name
  • Upstate
DescriptionHeLa + EGF Stimulated Cell Lysate in Mg2+ Lysis Buffer
OverviewEGF stimulated HeLa cell lysate. The cell lysate was quantitated via BCA assay. This sell lysate demonstrated higher levels of Ras-GTP form present as compared to unstimulated HeLa cell lysates. The assay was performed using the Ras GTPase Activation ELISA Kit (Catalog # 17-424)
References
Product Information
Presentation200 &micor;g of EGF stimulated HeLa cell lysate, in 40 µL of 1X MLB (Catalog 20-168) containing, 1 &micor;g/mL leupeptin, 1µg/mL aprotinin, 1 µg/mL pepstatin, 1mM PMSF.
Quality LevelMQ100
Applications
Key Applications
  • Western Blotting
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at -80C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap. Use immediately in assay following thawing as Ras GTP form can rapidly hydrolyze to the inactive Ras GDP form.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
12-500 04053252373480

Documentation

HeLa + EGF Stimulated Cell Lysate in Mg2+ Lysis Buffer SDS

Title

Safety Data Sheet (SDS) 

HeLa + EGF Stimulated Cell Lysate in Mg2+ Lysis Buffer Certificates of Analysis

TitleLot Number
HeLa + EGF Stimulated Cell Lysate in MLB 2978905
HeLa + EGF Stimulated Cell Lysate in MLB - 2137153 2137153
HeLa + EGF Stimulated Cell Lysate in MLB - 2436790 2436790
HeLa + EGF Stimulated Cell Lysate in MLB - 1957273 1957273
HeLa + EGF Stimulated Cell Lysate in MLB - 2038720 2038720
HeLa + EGF Stimulated Cell Lysate in MLB - 2318090 2318090
HeLa + EGF Stimulated Cell Lysate in MLB - 3231548 3231548
HeLa + EGF Stimulated Cell Lysate in MLB - 3319455 3319455
HeLa + EGF Stimulated Cell Lysate in MLB - 3442363 3442363
HeLa + EGF Stimulated Cell Lysate in MLB - 3735421 3735421