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AP112B Sigma-AldrichGoat Anti-Human IgG Antibody, biotin conjugate

This Goat anti-Human IgG Antibody, biotin conjugate is validated for use in ELISA, WB for the detection of Human IgG.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Species Reactivity: Human

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Overview

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Description
Catalogue Number	AP112B
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Goat Anti-Human IgG Antibody, biotin conjugate
Background Information	IgG is a monomeric immunoglobulin, consists of two heavy chains (gamma chains) and two light chains. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin. It is approximately equally distributed in blood and in interstitial fluid, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, providing protection to the fetus in its first weeks of life before its own immune system has developed. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

References

Product Information
Format	Biotin
Presentation	Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Goat anti-Human IgG Antibody, biotin conjugate is validated for use in ELISA, WB for the detection of Human IgG.
Key Applications	ELISA Western Blotting
Application Notes	EIA: 1:20,000-1:400,000 using enzyme-streptavidin conjugate. Western blots: 1:20,000-1:400,000 using enzyme-streptavidin conjugate. Immunohistochemistry: 1:500-1:5,000 using enzyme-streptavidin conjugate. Flow cytometry: 1:200-1:1,000. Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000. Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Prepared from purified human IgG.
Host	Goat
Specificity	The antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.
Species Reactivity	Human
Antibody Type	Polyclonal Antibody
Purification Method	ImmunoAffinity Purified

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.

Packaging Information
Material Size	2 mL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
AP112B	04053252478307

Documentation

Goat Anti-Human IgG Antibody, biotin conjugate SDS

Title
Safety Data Sheet (SDS)

Goat Anti-Human IgG Antibody, biotin conjugate Certificates of Analysis

Title	Lot Number
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED	2475585
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED SECONDARY ANTIBODY - 2117136	2117136
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 2492061	2492061
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3174897	3174897
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3212810	3212810
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3385800	3385800
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3427264	3427264
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3464588	3464588
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3488179	3488179
GOAT ANTI-HUMAN IgG BIOTIN CONJUGATED - 3574525	3574525

References

Reference overview	Pub Med ID
Herpes simplex virus type-2 specific glycoprotein G-2 immunomagnetically captured from HEp-2 infected tissue culture extracts. Charles R Clavet, Aaron B Margolin, Patrick M Regan Journal of virological methods 119 121-8 2004 Show Abstract Monoclonal antibody H1206 anti-HSV-2 gG-2 bound to tosylactivated paramagnetic Dynabeads (Dynal) has been used to isolate HSV-2 type-specific gG-2 from solubilized HEp-2 HSV-2 infected cell extracts. The immunomagnetically captured type-specific glycoprotein reacted strongly with monoclonal antibody H1206 and demonstrated a single band with apparent molecular weight of 100000 (100 kDa) and a doublet band with an apparent molecular weight of 60000-64000 (60-64 kDa). We observed the same exact banding pattern when monoclonal H1206 was immunoblotted with Helix pomatia lectin purified HSV-2 gG-2. The immunomagnetically purified gG-2 was unreactive to monoclonal antibody H1379 anti-HSV-1 gG-1 and four human HSV antibody negative sera. In addition, 20 human HSV antibody positive sera obtained from the Centers for Disease Control (CDC), Atlanta, GA, were used for the evaluation of our methodology. Immunoblotting of the human HSV antibody positive samples were in agreement with the CDC HSV serological designation. Sera characterized by reactivity to the immunomagnetically purified gG-2 in conjunction with Western blot has the potential to be used as a confirmatory serological test or to determine the accuracy of clinical serological immunoassays used to determine HSV-2 seropositivity.	15158593

Reference overview

Pub Med ID

Related Products & Applications

Alternative Format

Catalogue Number	Description
AP112	Goat Anti-Human IgG Antibody
AP112A	Goat Anti-Human IgG Antibody, Alkaline Phosphatase conjugate
AP112P	Goat Anti-Human IgG Antibody, HRP conjugate
AP112F	Goat Anti-Human IgG Antibody, FITC conjugate

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