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PF039 GDNF, Rat, Recombinant, S. frugiperda

GDNF, Rat, Recombinant, S. frugiperda MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rrGDNF, Glial Derived Neurotrophic Factor

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Overview

Replacement Information

Key Spec Table

Purity
≥97% by SDS-PAGE
Description
Overview

This product has been discontinued.



Recombinant, rat glial derived neurotrophic factor expressed in S. frugiperda insect cells. Native GDNF exists as a disulfide homodimeric glycoprotein and is a novel member of the TGF-β superfamily. Titrate the protein for optimal results in individual systems. Immbolized rrGFRa/Fc at 2 µg/ml (100 µl/neu) can bind GDNF at a linear range of 0.1-10 ng/ml.
Catalogue NumberPF039
Brand Family Calbiochem®
SynonymsrrGDNF, Glial Derived Neurotrophic Factor
References
ReferencesDurbec, P., et al. 1996. Nature 381, 789.
Jing, s., et al. 1996. Cell 85, 1113.
Treanor, J.J.S., et al. 1996. Nature 382 80.
Trupp, M., et al. 1996. Nature 381, 785.
Hudson, J., et al. 1995. Brain Res. Bull. 36, 425.
Tomac, A., et al. 1995. Nature 373, 335.
Lin, L.-F.H., et al. 1993. Science 260, 1130.
Product Information
FormLyophilized
FormulationLyophilized from 35% Acetonitrile, 0.1% TFA, 50 µg BSA/µg GDNF.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 1-3 ng/ml as measured by the ability to support the survival and stimulate neurite outgrowth of cultured embryonic chick dorsal root ganglia. Immbolized rrGFRα/Fc at 2 µg/ml (100 µl/neu) can bind GDNF at a linear range of 0.1-10 ng/ml.
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1 EU/µg GDNF
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PF039 0

Documentation

GDNF, Rat, Recombinant, S. frugiperda SDS

Title

Safety Data Sheet (SDS) 

GDNF, Rat, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
PF039

References

Reference overview
Durbec, P., et al. 1996. Nature 381, 789.
Jing, s., et al. 1996. Cell 85, 1113.
Treanor, J.J.S., et al. 1996. Nature 382 80.
Trupp, M., et al. 1996. Nature 381, 785.
Hudson, J., et al. 1995. Brain Res. Bull. 36, 425.
Tomac, A., et al. 1995. Nature 373, 335.
Lin, L.-F.H., et al. 1993. Science 260, 1130.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-September-2008 RFH
SynonymsrrGDNF, Glial Derived Neurotrophic Factor
DescriptionRecombinant, rat glial derived neurotrophic factor expressed in S. frugiperda insect cells. GDNF is a recombinant rat protein whose DNA sequence was inserted in a baculovirus expression vector and expressed in Sf 21 insect cells. The mature rat GDNF is a disulfide linked dimeric protein containing two 134 amino acid with a predicted molecular weight of 15 kDa for the monomer. Useful for proliferation studies. Glial cell line-derived neurotrophic factor (GDNF) was initially isolated based upon its ability to maintain embryonic midbrain dopaminergic neuron survival in vitro, and additional in vivo studies have provided support for the role of GDNF as a trophic factor in both the central and peripheral nervous systems. GDNF is structurally related to the TGF-β superfamily, and has been shown to interact with the GDNF receptor (GDNFRα). GDNFRα is associated with the cell membrane via a glycosyl-phosphatidylinositol (GPI) linkage, and together with Ret forms a functional receptor complex.
FormLyophilized
FormulationLyophilized from 35% Acetonitrile, 0.1% TFA, 50 µg BSA/µg GDNF.
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: ≤1 EU/µg GDNF
Biological activityED₅₀ = 1-3 ng/ml as measured by the ability to support the survival and stimulate neurite outgrowth of cultured embryonic chick dorsal root ganglia. Immbolized rrGFRα/Fc at 2 µg/ml (100 µl/neu) can bind GDNF at a linear range of 0.1-10 ng/ml.
SolubilityReconstitute in sterile PBS containing ≥0.1% HSA or BSA to a final concentration of ≥ 10 µg/ml.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C.
Toxicity Standard Handling
ReferencesDurbec, P., et al. 1996. Nature 381, 789.
Jing, s., et al. 1996. Cell 85, 1113.
Treanor, J.J.S., et al. 1996. Nature 382 80.
Trupp, M., et al. 1996. Nature 381, 785.
Hudson, J., et al. 1995. Brain Res. Bull. 36, 425.
Tomac, A., et al. 1995. Nature 373, 335.
Lin, L.-F.H., et al. 1993. Science 260, 1130.