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341590 FGF, Basic, Rat, Recombinant

FGF, Basic, Rat, Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: HBGF-2

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Overview

Replacement Information

Key Spec Table

Purity
≥95% by SDS-PAGE
Description
OverviewRecombinant, rat fibroblast growth factor (FGF) basic consisting of amino acids 11-154 and expressed in E. coli. FGF basic, a member of the FGF family of mitogenic peptides, is known to bind heparine or cell surface heparin sulfate proteoglycans for binding, dimerization, and activation of tyrosine kinase FGF receptors. Also known to bind polysaccharides, proteins, and lipids with lower affinity.
Catalogue Number341590
Brand Family Calbiochem®
SynonymsHBGF-2
References
ReferencesPresta, M., et al. 2005. Cytokine Growth Factor Rev. 16, 159.
Quarto, N., et al. 2005. Gene 356, 49.
Tsuneto, M., et al. 2005. Biochem. Biophys. Res. Commun. 335, 1239.
Claus, P., et al. 2003. J. Biol. Chem. 278, 479.
Coulier, F., et al. 1997. J. Mol. Evol. 44, 43.
Fernig, D., et al. 1994. Prog. Growth Factor Res. 5, 353.
Shimasaki, S., et al. 1988. Biochem. Biophys. Res. Commun. 157, 256.
Product Information
FormSolid
FormulationLyophilized from 0.2 µm filtered MOPS, Na₂SO₄, EDTA, DTT, BSA.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 0.3-1.8 ng/ml as measured in a cell proliferation assay using NR6R-3T3 mouse fibroblasts.
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
341590 0

Documentation

FGF, Basic, Rat, Recombinant SDS

Title

Safety Data Sheet (SDS) 

FGF, Basic, Rat, Recombinant Certificates of Analysis

TitleLot Number
341590

References

Reference overview
Presta, M., et al. 2005. Cytokine Growth Factor Rev. 16, 159.
Quarto, N., et al. 2005. Gene 356, 49.
Tsuneto, M., et al. 2005. Biochem. Biophys. Res. Commun. 335, 1239.
Claus, P., et al. 2003. J. Biol. Chem. 278, 479.
Coulier, F., et al. 1997. J. Mol. Evol. 44, 43.
Fernig, D., et al. 1994. Prog. Growth Factor Res. 5, 353.
Shimasaki, S., et al. 1988. Biochem. Biophys. Res. Commun. 157, 256.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2011 RFH
SynonymsHBGF-2
DescriptionRecombinant, rat fibroblast growth factor (FGF) basic consisting of amino acids 11-154 and expressed in E. coli. FGF basic, a member of the FGF family of mitogenic peptides, is known to bind heparine or cell surface heparin sulfate proteoglycans for binding, dimerization, and activation of tyrosine kinase FGF receptors. Also known to bind polysaccharides, proteins, and lipids with lower affinity.
FormSolid
FormulationLyophilized from 0.2 µm filtered MOPS, Na₂SO₄, EDTA, DTT, BSA.
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: <1.0 EU/µg protein
Biological activityED₅₀ = 0.3-1.8 ng/ml as measured in a cell proliferation assay using NR6R-3T3 mouse fibroblasts.
SolubilityReconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesPresta, M., et al. 2005. Cytokine Growth Factor Rev. 16, 159.
Quarto, N., et al. 2005. Gene 356, 49.
Tsuneto, M., et al. 2005. Biochem. Biophys. Res. Commun. 335, 1239.
Claus, P., et al. 2003. J. Biol. Chem. 278, 479.
Coulier, F., et al. 1997. J. Mol. Evol. 44, 43.
Fernig, D., et al. 1994. Prog. Growth Factor Res. 5, 353.
Shimasaki, S., et al. 1988. Biochem. Biophys. Res. Commun. 157, 256.