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341591 FGF-1, Acidic, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rhaFGF, Fibroblast Growth Factor, Acidic, Human, Recombinant, E. coli

341591
  
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Overview

Replacement Information

Key Spec Table

Purity
≥97% by SDS-PAGE
Description
Overview

This product has been discontinued.





Recombinant, human acidic fibroblast growth factor expressed in E. coli. Stimulates the proliferation of primary cultures of myoblasts and osteoblastic cells. Prevents myocardial apoptosis triggered by ischemia reperfusion injury. Exhibits mitogenic activity in vitro for cells or meso- and neuroectodermal origin. Its activity is greatly potentiated by addition of heparin.
Catalogue Number341591
Brand Family Calbiochem®
SynonymsrhaFGF, Fibroblast Growth Factor, Acidic, Human, Recombinant, E. coli
References
ReferencesCuevas, P., et al. 1997. Eur. J. Med. Res. 2, 465.
Junttila, T., et al. 1996. Brain Res. 707, 81.
Baird, A., et al. 1994. Curr. Opin. Neuro. Biol. 4, 78.
Gospodarowicz, D. 1991. Ann. N.Y. Acad. Sci. 638, 1.
Rizzino, A., et al. 1988. Cancer Res. 48, 4266.
Giminez-Gallego, G., et al. 1986. Biochem. Biophys. Res. Commun. 138, 611.
Harper, J.W., et al. 1986. Biochemistry 25, 4097.
Jaye, M., et al. 1986. Science 233, 541.
Thomas, K.A., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 357.
Product Information
CAS number106096-92-8
FormSolid
FormulationLyophilized from a sterile-filtered solution of 20 mM Tris, 1 M NaCl, 50 µg BSA/µg FGF acidic, pH 7.0.
ReconstitutionReconstitute to a concentration of ≥1 μg/ml with sterile PBS containing at least 0.1% HSA or BSA. Reconstituted material can be stored at +4°C for up to 3 months without detectable loss of activity. For longer storage, aliquot and freeze at -70°C. Avoid freeze/thaw cycles of solutions.
Applications
Biological Information
Biological activityED₅₀ = 100-300 pg/ml in the presence of 10 µg/ml heparin as monitored in a mitogenic assay by measuring the acidic FGF-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts
Purity≥97% by SDS-PAGE
Physicochemical Information
Contaminantsendotoxin: ≤ 1 EU/µg FGF
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSLK3641300
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at 4°C or for up to 6 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
341591 0

Documentation

FGF-1, Acidic, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
341591

References

Reference overview
Cuevas, P., et al. 1997. Eur. J. Med. Res. 2, 465.
Junttila, T., et al. 1996. Brain Res. 707, 81.
Baird, A., et al. 1994. Curr. Opin. Neuro. Biol. 4, 78.
Gospodarowicz, D. 1991. Ann. N.Y. Acad. Sci. 638, 1.
Rizzino, A., et al. 1988. Cancer Res. 48, 4266.
Giminez-Gallego, G., et al. 1986. Biochem. Biophys. Res. Commun. 138, 611.
Harper, J.W., et al. 1986. Biochemistry 25, 4097.
Jaye, M., et al. 1986. Science 233, 541.
Thomas, K.A., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 357.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-May-2008 RFH
SynonymsrhaFGF, Fibroblast Growth Factor, Acidic, Human, Recombinant, E. coli
DescriptionRecombinant, human acidic fibroblast growth factor expressed in E. coli. Composed of 140 amino acid residues. Stimulates the proliferation of primary cultures of osteoblastic cells. Found in brain, retina, bone matrix, and in osteosarcoma cells. Exhibits mitogenic activity in vitro for cells of meso- and neuroectodermal origin. Has a role in wound healing, induction of neuron differentiation, and modulation of embryonic development and differentiation. Acts as a chemoattractant and has mitogenic activity in vitro for endothelial cells and angiogenic activity in vivo. Activity is greatly potentiated by the addition of heparin.
FormSolid
FormulationLyophilized from a sterile-filtered solution of 20 mM Tris, 1 M NaCl, 50 µg BSA/µg FGF acidic, pH 7.0.
CAS number106096-92-8
RTECSLK3641300
Purity≥97% by SDS-PAGE
Contaminantsendotoxin: ≤ 1 EU/µg FGF
Biological activityED₅₀ = 100-300 pg/ml in the presence of 10 µg/ml heparin as monitored in a mitogenic assay by measuring the acidic FGF-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts
SolubilityReconstitute to ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at 4°C or for up to 6 months at -70°C.
Toxicity Standard Handling
Merck USA index14, 4072
ReferencesCuevas, P., et al. 1997. Eur. J. Med. Res. 2, 465.
Junttila, T., et al. 1996. Brain Res. 707, 81.
Baird, A., et al. 1994. Curr. Opin. Neuro. Biol. 4, 78.
Gospodarowicz, D. 1991. Ann. N.Y. Acad. Sci. 638, 1.
Rizzino, A., et al. 1988. Cancer Res. 48, 4266.
Giminez-Gallego, G., et al. 1986. Biochem. Biophys. Res. Commun. 138, 611.
Harper, J.W., et al. 1986. Biochemistry 25, 4097.
Jaye, M., et al. 1986. Science 233, 541.
Thomas, K.A., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 357.