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330657 Estrogen Receptorβ, Human, Recombinant, S. frugiperda

Estrogen Receptor β, Human, Recombinant, S. frugiperda, supplied as a purified, soluble, and functionally active protein with post-translational modifications similar to those found in human cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
330657
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, human estrogen receptor-β expressed in S. frugiperda insect cells. A hormone-inducible transcription factor that shares high homology with ER-α, especially in the DNA binding protein domain, suggesting that both receptors interact with related DNA response elements. Supplied as a purified, soluble, and functionally active protein with post-translational modifications similar to those found in human cells.
Aliquot in individual vials for daily use. Do not add any dilution buffers, as these may cause aggregation of the ER.
Catalogue Number330657
Brand Family Calbiochem®
References
ReferencesKuiper, G.G., et al. 1997. Endocrinology 138, 863.
Paech, K., et al. 1997. Science 277, 1508.
Mosselman, S., et al. 1996. FEBS Lett. 392, 49.
Obourn, J.D., et al. 1993. Biochemistry 32, 6229.
Product Information
Unit of DefinitionOne unit is defined as the amount of estrogen receptor that will bind 1.0 pmol ³H-estradiol overnight at 4°C as measured by quantitation of estradiol:receptor complexes in a hydroxylapatite assay.
FormLiquid
FormulationIn 400 mM KCl, 50 mM Bis-Tris-propane, 2 mM DTT, 1 mM EDTA, 10% glycerol, pH 9.0. DO NOT VORTEX.
Applications
Biological Information
Purity≥80% by SDS-PAGE
Specific Activity≥5000 units/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Do not add any dilution buffers, as these may cause aggregation of the ER.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
330657 0

Documentation

Estrogen Receptorβ, Human, Recombinant, S. frugiperda SDS

Title

Safety Data Sheet (SDS) 

Estrogen Receptorβ, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
330657

References

Reference overview
Kuiper, G.G., et al. 1997. Endocrinology 138, 863.
Paech, K., et al. 1997. Science 277, 1508.
Mosselman, S., et al. 1996. FEBS Lett. 392, 49.
Obourn, J.D., et al. 1993. Biochemistry 32, 6229.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-September-2007 JSW
DescriptionRecombinant, human estrogen receptor-β expressed in S. frugiperda insect cells. A hormone-inducible transcription factor that shares high homology with ER-α, especially in the DNA binding domain, suggesting that both receptors interact with related DNA response elements. Supplied as a purified, soluble, and functionally active protein with post-translational modifications similar to those found in human cells.
FormLiquid
FormulationIn 400 mM KCl, 50 mM Bis-Tris-propane, 2 mM DTT, 1 mM EDTA, 10% glycerol, pH 9.0. DO NOT VORTEX.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥80% by SDS-PAGE
Specific activity≥5000 units/mg protein
Unit definitionOne unit is defined as the amount of estrogen receptor that will bind 1.0 pmol ³H-estradiol overnight at 4°C as measured by quantitation of estradiol:receptor complexes in a hydroxylapatite assay.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Do not add any dilution buffers, as these may cause aggregation of the ER.
Toxicity Standard Handling
ReferencesKuiper, G.G., et al. 1997. Endocrinology 138, 863.
Paech, K., et al. 1997. Science 277, 1508.
Mosselman, S., et al. 1996. FEBS Lett. 392, 49.
Obourn, J.D., et al. 1993. Biochemistry 32, 6229.