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PMEF-CFL-P1 EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Strain CF1, Not Treated, Passage 1

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
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PMEF-CFL-P1
1 vial  1x106 cells
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Key Spec Table

Key Applications
Cult
Description
Catalogue NumberPMEF-CFL-P1
Trade Name
  • Embryomax
  • Specialty Media
DescriptionEmbryoMax® Primary Mouse Embryonic Fibroblasts
OverviewPMEF-CFL-P1 are low passage Primary Mouse Embryo Fibroblasts (PMEFs) isolated from CF1 mouse embryos at day 13. The cells have NOT been mitotically arrested. PMEFs are the traditional feeder cells used to support the growth of undifferentiated mouse and human pluripotent stem cell cultures. Additionally, low passage primary cultures of fibroblasts have a high proliferative capacity and can be used for reprogramming. It is known that cellular reprogramming efficiency declines with each passage, presumably due to lowered proliferative capacity of PMEFs at later passages. These untreated PMEF cells are high quality, low passage (P1) mouse fibroblasts that have been successfully reprogrammed into iPS cells with high efficiency. They can be used to study the mechanisms of reprogramming or be further expanded and treated with γ-irradiation or mitomycin-C to make growth arrested feeder cells. See Murine Embryonic Stem Cell Culture Manual for complete culture and media for PMEF cells.
Alternate Names
  • MEF Feeder Cells, CF-1 PMEFs, CF-1 MEFs, MEFs, Mouse Feeder Cells
Background InformationThe EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
References
Product Information
PresentationProvided frozen in EMD Millipore Freezing Medium (S-002-D) containing DMSO.
Quality LevelMQ100
Applications
ApplicationPMEF, Strain CF1, Not Treated, Passage 1
Key Applications
  • Stem Cell Culture
Biological Information
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • PMEFs
  • Induced Pluripotent Stem Cells
Cell Line Type
  • Primary Mouse Embryo Fibroblasts
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsAfter receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
Packaging Information
Material Size1 vial
Material Package1x106 cells
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PMEF-CFL-P1 04053252851711

Documentation

EmbryoMax® Primary Mouse Embryonic Fibroblasts SDS

Title

Safety Data Sheet (SDS) 

EmbryoMax® Primary Mouse Embryonic Fibroblasts Certificates of Analysis

TitleLot Number
EmbryoMax® PMEF P1 Strain CF-1, Untreated - 2389219 2389219
EmbryoMax® PMEF P1 -2492810 2492810
EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3133227 3133227
EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3153783 3153783
EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3178415 3178415
EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3438076 3438076
EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3681329 3681329
EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3796896 3796896
EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3801061 3801061
EmbryoMax® PMEF P1 Strain CF-1, Untreated - 3924980 3924980
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