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L6143-BC Easycoll, Density 1.124 g/ml

L6143-BC
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
OverviewThe basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). Our ready-to-use preparations are already adjusted to physiological values of osmolality and pH. Any desired densities from Easycoll (cat. no. L6143/5) can conveniently be prepared with PBS (cat. no. L1825).
Specifications:

Sterility: Pass
pH: 7.02-7.62
Osmolality: 0.281-0.310 Osm/kg H20
Density: 1.123-1.125 g/ml
Color: Light Yellow
Appearance: Clear
Separating solutions
Separating solutions are used for the isolation of cells or cellular particles during density gradient centrifugation. We offer two different separating solutions: Biocoll and Easycoll. These two solutions differ in the respectively contained polymer. Biocoll separating solution does contain a polymer with a molecular weight of approx. 400000 Dalton. The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). The ready-made solutions are already preset on physiological values of osmolality and ph-value.

Easycoll
The basis of Easycoll is silica gel particles, coated with PVP (polyvinyl pyrrolidone). Biochrom AG ready-to-use preparations are already adjusted to physiological values of osmolality and pH. Any desired densities from Easycoll (cat. no. L6143/5) can conveniently be prepared with PBS (cat. no. L1825).

Production of Easycoll with different densities
The ready-to-use separating solution Easycoll is already adjusted to physiological conditions of osmolality and pH value.
But you can also adjust a required density yourself by diluting Easycoll correspondingly.
The table below shows an example for different densities. All amounts are given for a temperature of +20 °C. Only PBS w/o Ca2+, Mg2+ is suitable, since these ions might influence agglomeration and hence separation of cell clusters.
Tab. 37: Instructions on how to dilute the stock solution of Easycoll (density 1.124 g/ml; cat. no. L6143/L6145) with PBS (cat. no. L1825/0)
See Table
Preparation of solutions with other densities can be made by isoosmolar dilution according to the following formula:
See figure
For the dilution we recommend the use of PBS without Ca2+, Mg2+, since both ions can influence the agglomeration of cells and consequently cell separation.
Catalogue NumberL6143-BC
Brand Family Calbiochem®
References
Product Information
Quality LevelMQ300
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
L6143-BC 0

Documentation