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219498 Cathepsin S Substrate, Fluorogenic - Calbiochem

An internally quenched, highly cathepsin S-selective, fluorogenic peptide substrate that is efficiently cleaved by CatS, but not by Cathepsin B & L.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Mca-GRWPPMG~LPWEK(Dnp)-D-R-NH2

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Overview

Replacement Information
Description
Overview

This product has been discontinued.



An internally quenched, highly cathepsin S-selective, fluorogenic peptide substrate that is efficiently cleaved by CatS (kcat/Km = 1.018 µM-1 S-1), but not by Cathepsin B & L (kcat/Km = 0.004 and 0.032 µM-1 S-1, respectively). Studies using CatS-specific inhibitor LHVS reveal that the substrate is not cleaved by other proteolytic enzymes, including CatB, CatH, CatL, and CatX, present in endosomal extracts from human B cell line WT100, dendritic cells, and macrophages. (excitation maximum: 340 nm; emission maximum: 405 nm)
Catalogue Number219498
Brand Family Calbiochem®
SynonymsMca-GRWPPMG~LPWEK(Dnp)-D-R-NH2
References
ReferencesLutzner, N., and Kalbacher, H. 2008. J. Biol. Chem. 283, 36185.
Product Information
FormYellow solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₉₃H₁₂₃N₂₅O₂₃S
Chemical formulaC₉₃H₁₂₃N₂₅O₂₃S
Hygroscopic Hygroscopic
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Peptide SequenceMca-Gly-Arg-Trp-Pro-Pro-Met-Gly~Leu-Pro-Trp-Glu-Lys(Dnp)-D-Arg-NH₂
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
219498 0

Documentation

Cathepsin S Substrate, Fluorogenic - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Cathepsin S Substrate, Fluorogenic - Calbiochem Certificates of Analysis

TitleLot Number
219498

References

Reference overview
Lutzner, N., and Kalbacher, H. 2008. J. Biol. Chem. 283, 36185.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-January-2011 RFH
SynonymsMca-GRWPPMG~LPWEK(Dnp)-D-R-NH2
DescriptionAn internally quenched, highly cathepsin S-selective, fluorogenic peptide substrate that is efficiently cleaved by CatS (kcat/Km = 1.018 µM-1 S-1), but not by Cathepsin B & L (kcat/Km = 0.004 and 0.032 µM-1 S-1, respectively). Studies using CatS-specific inhibitor LHVS reveal that the substrate is not cleaved by other proteolytic enzymes, including CatB, CatH, CatL, and CatX, present in endosomal extracts from human B cell line WT100, dendritic cells, and macrophages (excitation maximum: 340 nm; emission maximum: 405 nm).
FormYellow solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₉₃H₁₂₃N₂₅O₂₃S
Peptide SequenceMca-Gly-Arg-Trp-Pro-Pro-Met-Gly~Leu-Pro-Trp-Glu-Lys(Dnp)-D-Arg-NH₂
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml)
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesLutzner, N., and Kalbacher, H. 2008. J. Biol. Chem. 283, 36185.