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208713 Calpain-1, Human Erythrocytes

Calpain-1, Human Erythrocytes, is a native calpain-1. A heterodimeric cysteine proteinase with low Ca2+ requirement (EC₅₀ = 2 µM).

Calpain-1, Human Erythrocytes MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: μ-Calpain

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208713
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
208713-500UG Plastic ampoule 500 μg
Description
OverviewNative calpain-1 from human erythrocytes. Ca2+-dependent heterodimeric cysteine proteinase with low Ca2+ requirement (EC50= 2 µM).
Catalogue Number208713
Brand Family Calbiochem®
Synonymsμ-Calpain
References
ReferencesVanderklish, P.W., and Bahr, B.A. 2000. Int. J. Exp. Pathol. 81, 323.
Sorimachi, H., et al. 1997. Biochem. J. 328, 721.
Croall, D.E., and McGrody, K.S. 1994. Biochemistry 33, 13223.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 min, 25°C using the Calpain Activity Assay Kit, Fluorogenic (Cat. No. QIA120). Note: 1 caseinolytic unit = 1 fluorogenic unit.
EC number3.4.22.17
FormLiquid
FormulationIn 20 mM imidazole, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.
Quality LevelMQ100
Applications
Biological Information
PurityComparable to reference lot by SDS-PAGE
SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Specific Activity≥1000 units/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38-42/43

Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation and skin contact.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Harmful
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
208713-500UG 07790788048501

Documentation

Calpain-1, Human Erythrocytes SDS

Title

Safety Data Sheet (SDS) 

Calpain-1, Human Erythrocytes Certificates of Analysis

TitleLot Number
208713

References

Reference overview
Vanderklish, P.W., and Bahr, B.A. 2000. Int. J. Exp. Pathol. 81, 323.
Sorimachi, H., et al. 1997. Biochem. J. 328, 721.
Croall, D.E., and McGrody, K.S. 1994. Biochemistry 33, 13223.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-January-2009 JSW
Synonymsμ-Calpain
DescriptionNative calpain-1 from human erythrocytes. Ca2+-dependent cysteine proteinase with low Ca2+ requirement (half-maximal activation = 2 µM). Participates in the ATP release reaction of platelets stimulated with thrombin.
FormLiquid
FormulationIn 20 mM imidazole, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8.
Concentration Label Please refer to vial label for lot-specific concentration
SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
EC number3.4.22.17
PurityComparable to reference lot by SDS-PAGE
Specific activity≥1000 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 min, 25°C using the Calpain Activity Assay Kit, Fluorogenic (Cat. No. QIA120). Note: 1 caseinolytic unit = 1 fluorogenic unit.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Harmful
ReferencesVanderklish, P.W., and Bahr, B.A. 2000. Int. J. Exp. Pathol. 81, 323.
Sorimachi, H., et al. 1997. Biochem. J. 328, 721.
Croall, D.E., and McGrody, K.S. 1994. Biochemistry 33, 13223.