Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

SCT203 BioTracker™ Orange Zymosan Phagocytosis Live Cell Probe

This live cell imaging probe is zymosan conjugated to fluorescent orange dye & can be used to detect phagocytosis using fluorescence microscopy or flow cytometry.

SCT203
1 mg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT203
Data setup Error. Pagelet <feature_brand-BioTracker> not found.
DescriptionBioTracker™ Orange Zymosan Phagocytosis Live Cell Probe
OverviewZymosan A is a compound prepared from yeast cell walls and commonly used to induce inflammatory responses, especially phagocytosis. BioTracker™ Orange Zymosan Phagocytosis Live Cell Probe is Zymosan A labeled with an orange acidic pH indicator.

The fluorescence of this indicator is especially enhanced in an acidic environment such as that found in phagosomes or other acidic organelles. SCT203 is therefore a useful probe for the observaton of phagocytosis via its fluorescence increase, which can be only observed after phagocytosis. This probe emits fluorescence when excited by 532 or 514 nm sources, in addition to others such as the 488 laser that are compatible with the excitation range.

Spectral Properties
Absorbance (max): 535 nm
Emission (max): 568 nm (Orange)
Alternate Names
  • Live Cell Imaging Probe
  • Fluorescent Zymosan Probe
  • orange zymosan
  • orange zymosan live cell probe
Background InformationZymosan A is a compound prepared from yeast cell walls that is frequently used to induce inflammatory responses, including phagocytosis. SCT203 is Zymosan A labeled with AcidiFluor ORANGE, an acid pH indicator. Its fluorescence is enhanced in the acidic environment of the phagosome. BioTracker™ Orange Zymosan Phagocytosis Live Cell Probe is therefore a useful tool for the detection of the acidified conditions that follow phagocytosis.
References
Product Information
Detection methodFluorescence Flow cytometry
FormatLyophilized solid
PresentationReddish-purple solid
Quality LevelMQ100
Applications
ApplicationThis live cell imaging probe is zymosan conjugated to fluorescent orange dye & can be used to detect phagocytosis using fluorescence microscopy or flow cytometry.
Key Applications
  • Cell Based Assays
  • Flow Cytometry
  • Fluorescence
Application NotesThis fluorescently-labeled zymosan-A enables fluorescent imaging of phagocytic cells performing phagocytosis.

The lyophilized probe may be reconstituted in culture medium to 10 to 50 ug/mL, but concentration should be optimized to the user's application.

SCT203 has been used to analyze phagocytosis by fluorescence microscopy or flow cytometry in overnight-cultured RAW264.7 cells after incubation for 1-4h.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker™ Orange Zymosan Phagocytosis Live Cell Probe at -20°C, desiccated and protected from light. Centrifuge vial briefly to collect contents at bottom of vial before opening. Storing as a solution is not recommended.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCT203 04065269254249

Documentation