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OP07A Anti-v-Src (Ab-1) Mouse mAb (327) Agarose Conjugate

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP07A
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~60 kDa v-Src and c-Src proteins in NIH/3T3 and HT1080 cells. May appear as a doublet due to phosphorylation.
Catalogue NumberOP07A
Brand Family Calbiochem®
References
ReferencesCourtneidge, S.A., and Heber, A. 1987. Cell 50, 1031.
Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
Courtneidge, S.A. 1985. EMBO J. 4, 1471.
Whitman, M., et al. 1985. Nature 315, 239
Bolen, J.B., et al. 1984. Cell 38, 767.
Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
Collett, M.S., et al. 1978. Cell 15, 1363.
Product Information
FormLiquid suspension
FormulationIn PBS.
Negative controlHT1080 or NIH/3T3 cells
Positive controlCOLO-201 cells
Preservative≤0.1% sodium azide
Applications
Application ReferencesImmune Kinase Reaction Bolen, J.B., et al. 1985. Proc. Natl. Acad. Sci. 82, 7275. Immunoblotting Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Immunoprecipitation Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Original Clone Lipsich, L.A., et al. 1983. J. Virol. 48, 352.
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (Use Cat. No. OP07 or OP07L, see application references)
Immunoprecipitation (15 µl beads per sample, see application references)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Paraffin Sections (not recommended)
Application Commentspp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active Src that can be used for in vitro kinase assays (see application references. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified v-Src
ImmunogenHuman
Epitopewithin the SH3 domain of v-Src and c-Src
Clone327
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP07A 0

Documentation

Anti-v-Src (Ab-1) Mouse mAb (327) Agarose Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-v-Src (Ab-1) Mouse mAb (327) Agarose Conjugate Certificates of Analysis

TitleLot Number
OP07A

References

Reference overview
Courtneidge, S.A., and Heber, A. 1987. Cell 50, 1031.
Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
Courtneidge, S.A. 1985. EMBO J. 4, 1471.
Whitman, M., et al. 1985. Nature 315, 239
Bolen, J.B., et al. 1984. Cell 38, 767.
Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
Collett, M.S., et al. 1978. Cell 15, 1363.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-October-2007 RFH
ApplicationImmunoblotting (Use Cat. No. OP07 or OP07L, see application references)
Immunoprecipitation (15 µl beads per sample, see application references)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Paraffin Sections (not recommended)
DescriptionPurified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells (see application references). Conjugated to agarose. Recognizes the ~60 kDa Src protein.
BackgroundThe major translational product of the avian sarcoma virus has been designated pp60src. Avian sarcoma virus pp60src possesses a characteristic protein kinase activity with specificity for tyrosine residues. The mammalian cellular homologue of pp60src, designated pp60c-src, exhibits a similar protein tyrosine kinase activity which can be activated by interaction with polyoma virus-encoded middle T antigen. Immunoprecipitates of middle T antigen also contain phosphatidylinositol kinase activity coinciding with the presence of a 81 kDa (p81) protein. The p81 is phosphorylated by activated pp60c-src and is thought to be the phosphatidylinositol kinase.
HostMouse
Immunogen speciesHuman
Immunogenpurified v-Src
Epitopewithin the SH3 domain of v-Src and c-Src
Clone327
IsotypeIgG₁
Specieshuman, mouse, rat
Positive controlCOLO-201 cells
Negative controlHT1080 or NIH/3T3 cells
FormLiquid suspension
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
Commentspp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active Src that can be used for in vitro kinase assays (see application references. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesCourtneidge, S.A., and Heber, A. 1987. Cell 50, 1031.
Cartwright, C.A., et al. 1985. Mol. Cell. Biol. 5,, 2647.
Courtneidge, S.A. 1985. EMBO J. 4, 1471.
Whitman, M., et al. 1985. Nature 315, 239
Bolen, J.B., et al. 1984. Cell 38, 767.
Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci. 77, 1311.
Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci. 76, 3159.
Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci. 75, 2021.
Collett, M.S., et al. 1978. Cell 15, 1363.
Application referencesImmune Kinase Reaction Bolen, J.B., et al. 1985. Proc. Natl. Acad. Sci. 82, 7275. Immunoblotting Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Immunoprecipitation Grandori, C. and Hanfusa, H. 1988. J. Cell Biol. 107, 2125. Lipsich, L.A., et al. 1983. J. Virol. 48, 352. Original Clone Lipsich, L.A., et al. 1983. J. Virol. 48, 352.