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ABE1397 Anti-trimethyl STAT3 (Lys180) Antibody

This Anti-trimethyl STAT3 (Lys180) Antibody is validated for use in Western Blotting, Peptide Inhibition Assay, Immunoprecipitation for the detection of trimethyl STAT3 (Lys180).

Anti-trimethyl STAT3 (Lys180) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: STAT3, APRF UniProt Number: P40763
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ABE1397
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, PIA, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1397
DescriptionAnti-trimethyl STAT3 (Lys180) Antibody
Alternate Names
  • Signal transducer and activator of transcription 3
  • Acute-phase response factor
  • trimethyl STAT3 (Lys180)
Background InformationSignal transducer and activator of transcription 3 (UniProt P40763; also known as Acute-phase response factor, DNA-binding protein APRF) is encoded by the STAT3 (also known as ADMIO, APRF, HIES) gene (Gene ID 6774) in human. STAT proteins are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT3 is known to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). STAT3 gene knockout in mice is lethal at the early embryonic stage.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-trimethyl STAT3 (Lys180) Antibody is validated for use in Western Blotting, Peptide Inhibition Assay, Immunoprecipitation for the detection of trimethyl STAT3 (Lys180).
Key Applications
  • Western Blotting
  • Peptide Inhibition Assay
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: 5.0 µg from a representative lot immunoprecipitated STAT3 trimethylated on Lys180 in 0.5 mg of mouse embryonic stem cell lysate.
Peptide Inhibition Assay: Target specificity of a representative lot was confirmed by immunogen peptide blocking prior to Western blotting analysis (0.5 µg/mL) ) of mouse embryonic stem cell lysate (10 µg/lane) . Only the immunogen peptide, but not the corresponding peptide without the Lys180 trimethylation modification, blocked the detection of the target band.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the STAT3 K180me3 of human trimethyl STAT3 (Lys180)
EpitopeSTAT3 K180me3
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityTrimethylation site is present in all three human spliced isoforms. Reactivity toward Stat3 with mono- or dimethylated Lys180 has not been tested.
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human, Rat, Bovine, Chicken, Porcine, Orangatan based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • STAT3
  • APRF
Modifications
  • Methylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~88 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse embryonic stem cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected STAT3 trimethylated on Lys180 in 10 µg of mouse embryonic stem cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1397 04055977276923

Documentation

Anti-trimethyl STAT3 (Lys180) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-trimethyl STAT3 (Lys180) Antibody Certificates of Analysis

TitleLot Number
Anti-trimethyl STAT3 (Lys180) - 3228311 3228311
Anti-trimethyl STAT3 (Lys180) - 4014122 4014122
Anti-trimethyl STAT3 (Lys180) - 4201333 4201333
Anti-trimethyl STAT3 (Lys180) -Q2595870 Q2595870

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.