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MABN2678-25UG Anti-phospho-α-synuclein (Ser129) Antibody, clone PS129

Anti-phospho- -synuclein (Ser129), clone PS129, Cat. No. MABN2678, is a mouse monoclonal antibody that detects -synuclein and is tested for use in Western Blotting, Immunohistochemistry, and ELISA.

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Overview

Replacement Information
Description
Catalogue NumberMABN2678-25UG
DescriptionAnti-phospho-α-synuclein (Ser129) Antibody, clone PS129
Alternate Names
  • Non-A beta component of AD amyloid
  • Non-A4 component of amyloid precursor (NACP)
Background InformationAlpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP, PARK1) gene (Gene ID: 6622) in human. Alpha-synuclein is a membrane-bound protein in dopaminergic neurons that is involved in the regulation of dopamine release and transport. It is also reported to induce fibrillization of microtubule-associated protein tau and reduces neuronal responsiveness to various apoptotic stimuli. It is primarily expressed in the brain and is associated with the regulation of neurotransmitter release at synapses. Alpha-synuclein is also found in the cytoplasm, where it exists in a dynamic equilibrium between a soluble cytosolic form and a membrane-bound form. It associates with lipid membranes, particularly those of synaptic vesicles. Aggregation of alpha-synuclein is known to be a causative factor in the development of Parkinson's disease (PD), which leads to the degeneration of dopaminergic neurons in the substantia nigra (SN) in the midbrain region. -synuclein can be phosphorylated, predominantly on serine residues by casein kinase 1 (CK1). Phosphorylation by CK1 appears to occur on residue (serine 129) distinct from the residue (tyrosine 125) phosphorylated by other kinase FYN. The phosphorylation of -synuclein at serine 129 (pS129) has been extensively studied due to its significant implications in neurodegenerative diseases, particularly Parkinson's disease (PD) and other synucleinopathies. Phosphorylation of Ser129 is reported to be selective and extensive in synucleinopathy lesions and promotes insoluble fibril formation. This serves as a potential biomarker for disease diagnosis and progression and offers a promising target for therapeutic intervention. (Ref.: Vaikath, N.N., et al. (2015). Neurobiol Dis. 79:81-99; Majbour, N.K., et al. (2016). Mol Neurodegener. 19:11:7; El-Agnaf, O., et al. (2017). Neurobiol Dis. 104:85-96).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-phospho- -synuclein (Ser129), clone PS129, Cat. No. MABN2678, is a mouse monoclonal antibody that detects -synuclein and is tested for use in Western Blotting, Immunohistochemistry, and ELISA.
Key Applications
  • Western Blotting
  • ELISA
  • Immunohistochemistry
Application NotesTested Applications

ELISA Analysis: A representative lot detected -synuclein in ELISA application (Majbour, N.K., et al. (2016). Mol Neurodegener. 11:7; El-Agnaf, O., et al. (2017). Neurobiol Dis. 104: 85-96).

Immunohistochemistry Application: A representative lot detected phospho- -synuclein (Ser129) in Immunohistochemistry application (Vaikath, N.N., et al. (2015). Neurobiol Dis. 79:81-99).

Western Blotting Analysis: A representative lot detected phospho- -synuclein (Ser129) in Western Blotting application (Majbour, N.K., et al. (2016). Mol Neurodegener. 11:7).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 9 amino acids surrounding phosphoserine 129 from the C-terminal region of human -synuclein.
EpitopeC-terminal
ClonePS129
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone PS129 is a mouse monoclonal antibody that specifically detects -synuclein phosphorylated on serine 129.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat, Bovine, Porcine based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SNCA
Modifications
  • Phosphorylation
Purification MethodProtein G purified
UniProt Number
Molecular WeightTarget molecular weight ~16 kDa observed; 14.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant -synuclein phosphorylated S129 and recombinant -synuclein monomers.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected -synuclein phosphorylated on serine 129 in recombinant -synuclein phosphorylated S129 protein, but not in recombinant -synuclein monomers.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2678-25UG 04065272161480