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16-236 Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor® 555

Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor 555 is an antibody against phospho-erbB-2/HER-2 (Tyr1248) for use in FC & IC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors, Kinases & Phosphatases Gene Symbol: ERBB2, HER2, HER-2, NGL, herstatin, erbB-2, C-erbB-2, NEU, TKR1, p185erbB2, HER-2/neu UniProt Number: P04626
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RFC, ICCRbAlexaFluor®555Polyclonal Antibody
Description
Catalogue Number16-236
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor® 555
References
Product Information
FormatAlexaFluor®555
Quality LevelMQ100
Applications
ApplicationAnti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor 555 is an antibody against phospho-erbB-2/HER-2 (Tyr1248) for use in FC & IC.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
Biological Information
ImmunogenSynthetic phosphopeptide, residues 1243-1255, corresponding to a major autophosphorylation (Tyr1248) site of human erbB2/HER-2 (C-AENPEpYLGLDVPV)
HostRabbit
Specificityactivated form of erbB2/HER-2 oncoprotein
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Species Reactivity NotePredicted to cross-react with rat based on conservation of immunogenic sequence
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Gene Symbol
  • ERBB2
  • HER2
  • HER-2
  • NGL
  • herstatin
  • erbB-2
  • C-erbB-2
  • NEU
  • TKR1
  • p185erbB2
  • HER-2/neu
Modifications
  • Phosphorylation
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF- alpha and amphiregulin.
SIZE: 1255 amino acids; 137910 Da
SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity).
SIMILARITY: SwissProt: P04626 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunostaining of EGF treated A431 cells
Usage Statement
  • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at 4°C from date of shipment
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
16-236 04053252739576

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Alternative Format

Catalogue Number Description
06-229 Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody
16-235 Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor® 488

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies