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07-2119 Anti-phospho-RalA Antibody (Ser194)

Detect the phospho-RalA (Ser194) protein using this Anti-phospho-RalA (Ser194) validated for use in WB & Peptide Inhibition Assay.

Anti-phospho-RalA Antibody (Ser194) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Metabolism Research Sub-Category: Insulin/Energy Signaling Gene Symbol: RALA, Ral UniProt Number: P11233
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07-2119
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, B, R, Xn, ZebrafishWB, PIARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-2119
DescriptionAnti-phospho-RalA Antibody (Ser194)
Alternate Names
  • Ras-related protein Ral-A
Background InformationRalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes. RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarized exocytosis in epithelial cells.
References
Product Information
FormatAffinity Purified
Control
  • PANC10.05 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect the phospho-RalA (Ser194) protein using this Anti-phospho-RalA (Ser194) validated for use in WB & Peptide Inhibition Assay.
Key Applications
  • Western Blotting
  • Peptide Inhibition Assay
Application NotesWestern Blot Analysis: A representative lot detected phospho-RalA (Ser194) in certain primary and metastatic cell lysates, and in certain unmatched tumor tissue lysate samples.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human RalA phosphorylated at Ser194.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes RalA phosphorylated at Ser194.
Species Reactivity
  • Human
  • Bovine
  • Rat
  • Xenopus
  • Zebrafish
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Bovine, Rat, Xenopus, and Zebrafish based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RALA
  • Ral
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~25 kDa observed. Uncharacterized bands may be observed at ~20 kDa, 100 kDa, and 130-170 kDa in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in PANC10.05 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected phospho-RalA in PANC10.05 cell lysate. Peptide inhibition was observed with the addition of phospho peptides but not with the addition of non-phospho peptides.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-2119 04053252832338